PMID: 9557682Apr 29, 1998Paper

Cloning of novel isoforms of the human Gli2 oncogene and their activities to enhance tax-dependent transcription of the human T-cell leukemia virus type 1 genome

Journal of Virology
A TanimuraM Yoshida

Abstract

The expression of human T-cell leukemia virus type 1 (HTLV-1) is activated by interaction of a viral transactivator protein, Tax, and cellular transcription factor, CREB (cyclic AMP response element binding protein), which bind to a 21-bp enhancer in the long terminal repeats (LTR). THP (Tax-helping protein) was previously determined to enhance the transactivation by Tax protein. Here we report novel forms of the human homolog of a member of the Gli oncogene family, Gli2 (also termed Gli2/THP), an extended form of a zinc finger protein, THP, which was described previously. Four possible isoforms (hGli2 alpha, beta, gamma, and delta) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S, in the LTR. The longer isoforms, alpha and beta, were abundantly expressed in various cell lines including HTLV-1-infected T-cell lines. Fusion proteins of the hGli2 isoforms with the DNA-binding domain of Gal4 activated transcription when the reporter contained a Gal4-binding site and one copy of the 21-bp sequence, to which CREB binds. This activation was observed only in the presence of Tax. The 21-bp sequence in the reporter was also essential for the activation. These resu...Continue Reading

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Citations

Feb 13, 2001·Journal of Virology·M J SmithD M Markovitz
Apr 25, 2008·Journal of Clinical and Experimental Hematopathology : JCEH·Jia WangFusayoshi Murata
Oct 12, 2010·AIDS Research and Human Retroviruses·Filipe Ferreira de Almeida RegoLuiz Carlos Alcantara
Nov 12, 2013·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Qiushi JinXiaoping Li
Apr 5, 2020·Cell Communication and Signaling : CCS·Stephan J Matissek, Sherine F Elsawa
Feb 13, 2001·Journal of Virology·C M BrowningD M Markovitz
Mar 13, 2001·Annual Review of Immunology·M Yoshida
Oct 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Erich RoesslerMaximilian Muenke
Jun 6, 2003·The British Journal of Dermatology·M TojoF Kaneko
Mar 16, 2018·Molecular Biology of the Cell·Thibaut EguetherGregory J Pazour
Jan 27, 2021·International Journal of Molecular Sciences·Sidney IrianaNeelam Sharma-Walia
Nov 27, 1999·Current Biology : CB·C von Mering, K Basler
Sep 28, 1999·Trends in Genetics : TIG·A Ruiz i Altaba
Jan 18, 2006·Developmental Biology·Jennifer A CrokerRobert A Holmgren
Sep 23, 2000·American Journal of Human Genetics·E H VillavicencioP M Iannaccone

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