Cloning of novel transcripts of the human guanine-nucleotide-exchange factor Mss4: in situ chromosomal mapping and expression in pancreatic cancer

Genomics
F Müller-PillaschT M Gress

Abstract

In a previous large-scale analysis of gene expression in pancreatic cancer using gridded arrays of cDNA libraries and differential hybridizations, a gene that was a homolog to human mss4 was identified. Mss4 is a guanine-nucleotide-exchange factor for the Sec4/ Ypt1/Rab family of small GTP-binding proteins involved in the regulation of intracellular vesicular transport. By fluorescence in situ hybridization the human mss4 gene was assigned to chromosome 1q32-q41. Northern blot analysis revealed that three mss4 mRNA species are transcribed in human tissues of 780, 1200, and 2800 bp in length, respectively. Cloning and sequencing of the human mss4 transcripts from a pancreatic cancer cDNA library revealed that these mRNA species differ in the length of the 3-untranslated region and are probably due to the alternate use of polyadenylation sites. All mRNA species were detected at moderate to high levels in pancreatic cancer cell lines and were overexpressed in pancreatic cancer tissue compared to both normal pancreas and chronic pancreatitis tissue. However, the 1200-bp transcript was the Mss4 mRNA species with the highest level of expression in more than 50% of tumor cells and tissues. High levels of expression were found as well ...Continue Reading

References

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Citations

Mar 26, 2003·The American Journal of Pathology·Christine A Iacobuzio-DonahueMichael Goggins
Jul 12, 2005·Analytical and Bioanalytical Chemistry·Rajendra N Goyal, Anuradha Tyagi
Dec 29, 1999·Genes, Chromosomes & Cancer·J HuE J Perlman
Sep 14, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sachiko Toma-Fukai, Toshiyuki Shimizu
May 4, 2004·Molecular and Cellular Biology·Thomas V O HansenFinn C Nielsen

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