PMID: 3759912Oct 1, 1986Paper

Cloning of Pseudomonas sp. strain CBS3 genes specifying dehalogenation of 4-chlorobenzoate

Journal of Bacteriology
P SavardM Sylvestre

Abstract

The degradation of 4-chlorobenzoate (4-CBA) by Pseudomonas sp. strain CBS3 is thought to proceed first by the dehalogenation of 4-CBA to 4-hydroxybenzoate (4-HBA), which is then metabolized following the protocatechuate branch of the beta-ketoadipate pathway. The cloning of the 4-CBA dehalogenation system was carried out by constructing a gene bank of Pseudomonas sp. strain CBS3 in Pseudomonas putida. Hybrid plasmid pPSA843 contains a 9.5-kilobase-pair fragment derived from the chromosome of Pseudomonas sp. strain CBS3. This plasmid confers on P. putida the ability to dehalogenate 4-CBA and grow on 4-CBA as the only source of carbon. However, pPSA843 did not complement mutants of P. putida unable to grow on 4-HBA (POB-), showing that the genes involved in the metabolism of 4-HBA were not cloned. Subcloning of Pseudomonas sp. strain CBS3 genes revealed that most of the insert is required for the dehalogenation of 4-CBA, suggesting that more than one gene product is involved in this dehalogenation.

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Citations

Sep 1, 1992·FEMS Microbiology Reviews·M M Häggblom
Jan 1, 1990·Biodegradation·L C Commandeur, J R Parsons
Aug 9, 2003·The Journal of Biological Chemistry·James B ThodenHazel M Holden
Jan 1, 1991·Critical Reviews in Biotechnology·D J Hardman
Jan 1, 1992·Applied and Environmental Microbiology·A C LaytonG S Sayler
Dec 1, 1990·Applied and Environmental Microbiology·W J Hickey, D D Focht
May 7, 2003·Applied and Environmental Microbiology·Zhihao ZhuangDebra Dunaway-Mariano
Mar 1, 1991·Microbiological Reviews·G R Chaudhry, S Chapalamadugu
Mar 1, 1994·Applied and Environmental Microbiology·P W CoschiganoL Y Young
Dec 1, 1994·Microbiological Reviews·S Fetzner, F Lingens
Jul 12, 1991·Science·J D ScholtenD Dunaway-Mariano

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