Cloning of Russian sturgeon (Acipenser gueldenstaedtii) growth hormone and insulin-like growth factor I and their expression in male and female fish during the first period of growth.

Journal of Endocrinological Investigation
S Yom DinGad Degani

Abstract

In this study, the GH and IGF-I of the Russian sturgeon (rs), Acipenser gueldenstaedtii, were cloned and sequenced, and their mRNA gene expression determined. In addition, to improve our understanding of the GH function, the expression of this hormone was assessed in young males and females. Moreover, IGF-I expression was quantified in young males and compared to that in older ones. The nucleotide sequence of the rsGH cDNA was 980 bp long and had an open reading frame of 642 bp, beginning with the first ATG codon at position 39 and ending with the stop codon at position 683. A putative polyadenylation signal, AATAAA, was recognized 42 bp upstream of the poly (A) tail. The position of the signal- peptide cleavage site was predicted to be at position 111, yielding a signal peptide of 24 amino-acids (aa) and a mature peptide of 190 aa. When the rsGH aa sequence was compared with other species, the highest degree of identity was found to be with mammalians (66-70% identity), followed by anguilliformes and amphibia (61%) and other fish (39-47%). The level of rsGH mRNA was discovered to be similar in pituitaries of females and males of 5 age groups (1, 2, 3, 4, and 5- yr-old). In females and males, the levels did not change dramatica...Continue Reading

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Citations

Jan 17, 2014·Journal of Applied Genetics·Mahtab YarmohammadiAlireza Abbasalizadeh
Aug 9, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Gal LevyGad Degani
Apr 2, 2011·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Dalia David, Gad Degani
Dec 3, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Carlin M Fenn, Brian C Small
May 4, 2016·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Carlin M FennBrian C Small
Sep 18, 2020·Molecular and Cellular Endocrinology·Luis Fabián Canosa, Juan Ignacio Bertucci

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