PMID: 9657980Jul 11, 1998Paper

Cloning of subunits of convulxin, a collagen-like platelet-aggregating protein from Crotalus durissus terrificus venom

The Biochemical Journal
M Leduc, C Bon

Abstract

Convulxin (CVX) is a potent platelet-aggregating glycoprotein from the venom of the snake Crotalus durissus terrificus. It consists of two subunits, alpha and beta, joined by disulphide bridges in a hexameric structure. A cDNA library from venom gland was constructed in the vector pT3T7. The cloned cDNAs encoding the two chains of CVX were sequenced. Both are preceded by an identical 23-amino acid peptide signal sequence and encode sequences of 135 amino acids for the alpha chain and 125 amino acids for the beta chain. These polypeptides include a carbohydrate-recognition domain (CRD) in which some of the specific amino acids required for binding Ca2+ and galactose or mannose are absent. The presence of such a domain means that CVX can be included in the family of C-type lectins along with other snake venom proteins, although it is not a true lectin. Assuming that the localization of intracatenary disulphide bridges of each CVX chain is similar to that of the CRD and that an intercatenary bridge between the alpha and beta chains is similar to that of the C-type lectin botrocetin, we postulate the existence of an additional intercatenary bridge, which explains the tridimeric structure (alphabeta)3 of CVX.

Citations

Oct 13, 2012·Toxicon : Official Journal of the International Society on Toxinology·Ryan J R McCleary, R Manjunatha Kini
Mar 31, 2010·Toxicon : Official Journal of the International Society on Toxinology·Kenneth J Clemetson
Feb 24, 2009·Biochimie·Johara Boldrini-FrançaMaria I Homsi-Brandeburgo
Dec 9, 2008·Toxicon : Official Journal of the International Society on Toxinology·Jed JebaliAli Gargouri
Dec 8, 2004·Toxicon : Official Journal of the International Society on Toxinology·Tomohisa OgawaMotonori Ohno
Sep 29, 2004·Biochimica Et Biophysica Acta·Ene SiigurJüri Siigur
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·Takashi Morita
May 7, 2005·Toxicon : Official Journal of the International Society on Toxinology·Qiumin LuKenneth J Clemetson
Sep 27, 2012·Toxicon : Official Journal of the International Society on Toxinology·Jian FengChris Shaw
Jul 12, 2012·Toxicon : Official Journal of the International Society on Toxinology·Franziska T Arlinghaus, Johannes A Eble
Feb 15, 2002·The Journal of Biological Chemistry·Darren LockeMark L Kahn
Jul 26, 2003·The Journal of Biological Chemistry·Sachiko KanajiThomas J Kunicki
May 3, 2005·Biological & Pharmaceutical Bulletin·Shigeki SugawaraKazuo Nitta
May 13, 2006·Journal of Thrombosis and Haemostasis : JTH·K KatoT J Kunicki
May 10, 2001·The Journal of Biological Chemistry·R K AndrewsS P Watson
Aug 31, 2019·BioMed Research International·Bruno Tumang FrareLuiz Fernando Moreira Izidoro
Jul 8, 2020·Toxicon : Official Journal of the International Society on Toxinology·Lidia J TasimaAnita M Tanaka-Azevedo
Apr 25, 2000·Biochemical and Biophysical Research Communications·I M FrancischettiC Bon
Jun 30, 2000·Biochemical and Biophysical Research Communications·F NiedergangM Leduc
Oct 8, 1999·Biochemical and Biophysical Research Communications·C H ChungT F Huang
Jul 22, 1999·Toxicon : Official Journal of the International Society on Toxinology·H C CastroR B Zingali
Aug 8, 2002·Toxicon : Official Journal of the International Society on Toxinology·Alice M C MartinsHelena S A Monteiro
Sep 11, 2002·Toxicon : Official Journal of the International Society on Toxinology·Qin WeiYu-Liang Xiong
May 29, 2020·Journal of Medicinal Chemistry·Holly FosterRichard Foster

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