Cloning of the altered mRNA stability (ams) gene of Escherichia coli K-12.

Journal of Bacteriology
F Claverie-MartinS R Kushner

Abstract

A temperature-sensitive mutation in the ams gene of Escherichia coli causes an increase in the chemical half-life of pulse-labeled RNA at the nonpermissive temperature. Using lambda clones containing DNA fragments from the 23- to 24-min region on the E. coli chromosome, we have isolated a 5.8-kilobase DNA fragment which, when present in a low-copy-number plasmid, complements the conditional lethality and increased mRNA stability associated with the ams-1 mutation. The approximate initiation site and the direction of transcription of the ams gene were determined from the size of truncated polypeptides produced by Tn1000 insertions and Bal 31 deletions. Overexpression of the ams locus by using a T7 RNA polymerase-promoter system permitted the identification of an ams-encoded polypeptide of 110 kilodaltons.

References

Sep 9, 1977·Molecular & General Genetics : MGG·M KuwanoY Ohnishi
Jan 1, 1979·Journal of Bacteriology·A SancarW D Rupp
Dec 15, 1978·Journal of Molecular Biology·M S Guyer
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W P Donovan, S R Kushner
Sep 1, 1989·Journal of Bacteriology·C M HamiltonS R Kushner
Apr 1, 1985·Cell·M P Deutscher
Mar 1, 1986·Molecular & General Genetics : MGG·O FayetC Georgopoulos
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Jan 1, 1974·Journal of Supramolecular Structure·C P Georgopoulos, H Eisen
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J KarnG Cesareni
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Apr 1, 1980·Journal of Molecular Biology·M J Casadaban, S N Cohen

❮ Previous
Next ❯

Citations

Jan 1, 1992·Journal of Bacteriology·S K SrivastavaD Kennell
Feb 1, 1992·Journal of Bacteriology·M D HenryS R Kushner
Jan 1, 1993·Biochimie·A Miczak, D Apirion
Apr 1, 2007·EcoSal Plus·Sidney R Kushner
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.