Cloning of the biotin synthetase gene from Bacillus sphaericus and expression in Escherichia coli and Bacilli

Gene
I OhsawaK Kamogawa

Abstract

Biotin synthetase (BS) catalyses the biotransformation of dethiobiotin (DTB) to biotin. Here we report the cloning, characterization and expression of the gene encoding BS of Bacillus sphaericus. A recombinant plasmid pSB01, containing an 8.2-kb DNA fragment from B. sphaericus, was isolated by phenotypic complementation of an Escherichia coli bioB strain. Nucleotide sequence analysis of this fragment and N-terminal sequence determination of the recombinant protein product revealed that the bioB gene of B. sphaericus consists of a 996-bp open reading frame which is closely associated with at least one other gene. E. coli cells transformed with a bioB expression vector performed efficient bioconversion of DTB to biotin under defined culture conditions. Biotin production from transformed Bacillus subtilis and B. sphaericus recombinant strains was also demonstrated. Comparison of the amino acid sequences of BS from E. coli and B. sphaericus revealed extensive similarity.

References

Jan 5, 1979·Molecular & General Genetics : MGG·S Chang, S N Cohen
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 1, 1972·Journal of Bacteriology·P P Cleary, A Campbell
Aug 1, 1968·Journal of Bacteriology·B Rolfe, M A Eisenberg
Jan 1, 1983·Annual Review of Genetics·S NormarkO Olsson
Jul 1, 1982·Gene·E H Szybalski, W Szybalski
Jan 1, 1983·Methods in Enzymology·J Messing
Jan 1, 1984·Journal of General Microbiology·K O McDonald, W F Burke
Jun 5, 1983·Journal of Molecular Biology·D Hanahan

❮ Previous
Next ❯

Citations

Jul 29, 1994·Journal of Biotechnology·N SakuraiT Tosa
Dec 1, 2012·Nucleic Acids Research·Daniel H HaftErin Beck
Jul 18, 1997·Biochemical and Biophysical Research Communications·D Guianvarc'hA Marquet
Mar 22, 2002·The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi·Goro MatsumiyaHikaru Matsuda
Jan 1, 1992·Heart and Vessels. Supplement·O MatsubaraF Numano
Jan 1, 1992·Heart and Vessels. Supplement·J Amano, A Suzuki
Jan 1, 1993·DNA Sequence : the Journal of DNA Sequencing and Mapping·K HatakeyamaH Yukawa
Jan 1, 1991·Journal of Chemical Technology and Biotechnology·S W BrownK Hayakawa
Oct 1, 1993·Applied and Environmental Microbiology·N SakuraiT Tosa
Sep 1, 1993·Applied and Environmental Microbiology·N SakuraiT Tosa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.