PMID: 8945635Nov 1, 1996Paper

Cloning of the cDNA encoding mouse PP5/TFPI-2 and mapping of the gene to chromosome 6

DNA and Cell Biology
Y MiyagiK Miyazaki

Abstract

Placental protein 5 (PP5)/tissue factor pathway inhibitor-2 (TFPI-2) is a new homologue of TFPI, which contains three tandemly repeated Kunitz-type proteinase inhibitory (KPI) domains and potently inhibits the extrinsic blood coagulation cascade. In this study, mouse PP5/TFPI-2 cDNA was cloned using a human PP5/TFPI2 cDNA fragment as a probe. The characteristic three KPI domains with short spacer sequences and a basic amino acid stretch in the carboxyl-terminal region present in human PP5/TFPI-2 were well conserved in mouse PP5/TFPI-2. In general, the P1 reactive site residues of active KPI domains are basic amino acids. However, the putative P1 residues of the first, second, and third KPI domains were glutamine, aspartic acid, and serine, respectively. Mouse PP5/TFPI-2 mRNA was highly expressed in developing placenta as in humans. Adult liver and kidney also contained a significant amount of its transcripts. The mouse PP5/TFPI-2 gene was found to be located in the R-positive A2 band by the direct R-banding FISH and identified at 2.7 cM proximal to D6Mit 1 by interspecific backcross analysis.

References

Oct 28, 1977·Archiv für Gynäkologie·H Bohn, W Winckler
Oct 1, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·S I Rapaport, L V Rao
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B M MuellerW Ruf
Jan 1, 1992·Cytogenetics and Cell Genetics·Y MatsudaV M Chapman
May 1, 1992·Journal of Biochemistry·K EnjyojiH Kato
Sep 1, 1991·British Journal of Cancer·J C Murray
Nov 11, 1990·Nucleic Acids Research·R L WesselschmidtG J Broze
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M S BajajS P Bajaj
Aug 21, 1990·Biochemistry·G J BrozeW F Novotny
May 6, 1988·Biochimica Et Biophysica Acta·J L Manley
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G J Broze, J P Miletich
Jan 15, 1988·Biochemical and Biophysical Research Communications·R BützowM Seppälä
Feb 15, 1988·Biochemical and Biophysical Research Communications·L TakemotoR Wong
Jan 1, 1985·Archives of Gynecology·A MeisserH Bohn
Jul 5, 1985·Journal of Molecular Biology·G von Heijne
May 31, 1982·Life Sciences·J E SiiteriM Seppälä
Jan 1, 1980·Annual Review of Biochemistry·M Laskowski, I Kato
Jan 1, 1994·Seminars in Thrombosis and Hemostasis·W F Novotny
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C A SprecherD C Foster

❮ Previous
Next ❯

Citations

Aug 19, 2003·Archives of Biochemistry and Biophysics·Xin DuWalter Kisiel
Jun 4, 1998·International Journal of Cancer. Journal International Du Cancer·C N RaoD T Woodley
Apr 16, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Hisao Kato
Apr 14, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·H ItohM Koono

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.