Cloning of the citrate permease gene of Lactococcus lactis subsp. lactis biovar diacetylactis and expression in Escherichia coli.

Applied and Environmental Microbiology
F SesmaD de Mendoza

Abstract

The citrate plasmid (Cit+ plasmid) from Lactococcus lactis subsp. lactis biovar diacetylactis was cloned into the EcoRI site of plasmid pUC18. This recombinant plasmid enabled Escherichia coli K-12 to transport and utilize citrate as a source of energy, indicating expression of the citrate permease from L. lactis biovar diacetylactis. The citrate permease was under the control of the lac promoter of pUC18. Genetic expression of the Cit+ plasmid in maxicells revealed that the plasmid encoded two polypeptides of 47 and 32 kilodaltons, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

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Citations

Dec 4, 2012·Applied and Environmental Microbiology·Agata M Pudlik, Juke S Lolkema
May 28, 2014·FEMS Microbiology Reviews·Stuart AinsworthDouwe van Sinderen
Nov 6, 2007·Journal of Bacteriology·Matilde FernándezMercedes Maqueda
Oct 1, 1996·Antonie van Leeuwenhoek·M Cocaign-BousquetN D Lindley
Oct 1, 1996·The Journal of Applied Bacteriology·R M de FigueroaG Oliver
Jun 12, 2020·Frontiers in Microbiology·Jhonatan A Hernandez-ValdesOscar P Kuipers
May 24, 2017·Microorganisms·Valérie LarouteMarie-Line Daveran-Mingot
Mar 21, 1998·Applied and Environmental Microbiology·N García-QuintánsP López
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Iwona Sobczak, Juke S Lolkema
Dec 25, 2007·Applied and Environmental Microbiology·Claudia SánchezHelena Santos
Feb 5, 2008·Applied and Environmental Microbiology·Nieves García-QuintánsPaloma López

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