PMID: 8944755Nov 1, 1996Paper

Cloning of the gene encoding the antibacterial peptide drosocin involved in Drosophila immunity. Expression studies during the immune response

European Journal of Biochemistry
M CharletM Meister

Abstract

A potent inducible antibacterial peptide carrying an O-glycosylated substitution has recently been isolated from Drosophila [Bulet, P., Dimarcq, J. L., Hetru, C., Lagueux, M., Charlet, M., Hegy, G., Van Dorsselaer, A. and Hoffmann, J. A. (1993) J. Biol. Chem. 268, 14893-14897]. Here we report cloning studies that show that Drosophila contains a single, intronless gene, located at position 51C1-6, which encodes the precursor protein from which drosocin is processed. The upstream and the downstream sequences of the drosocin gene contain putative cis-regulatory elements similar to mammalian regulatory motifs, namely three kappa B-related decameric sequences. The drosocin gene is silent in naive animals, and is strongly induced with acute phase kinetics after immune challenge in larvae and in adults. We have established several transgenic fly lines in which reporter genes were placed under the control of various drosocin promoter sequences. Our results indicate that 2.5 kb of upstream sequences confer inducibility and tissue specificity to the transgene, but that the level of its expression in the fat body after immune challenge is low. Addition of genomic regions downstream of the drosocin transcribed sequences results in increase...Continue Reading

References

Nov 16, 1992·Biochemical and Biophysical Research Communications·C SamakovlisD Hultmark
Feb 15, 1992·European Journal of Biochemistry·Y TryseliusD Hultmark
Feb 26, 1991·European Journal of Biochemistry·S C SunI Faye
Jul 11, 1984·Nucleic Acids Research·P O O'Connell, M Rosbash
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·V CorcesM Meselson
Jan 1, 1982·Annals of the New York Academy of Sciences·I Kushner
Feb 1, 1995·Current Opinion in Immunology·J A Hoffmann
Jul 20, 1993·Journal of Molecular Biology·Y EngströmI Faye
Feb 1, 1996·Current Opinion in Immunology·J A HoffmannC Hetru

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Citations

Nov 9, 2000·Journal of Peptide Science : an Official Publication of the European Peptide Society·L Otvos
Dec 12, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M MeisterJ A Hoffmann
Jul 30, 1999·Developmental and Comparative Immunology·Y Engström
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Phoebe TzouBruno Lemaitre
Sep 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tania DottoriniFlaminia Catteruccia
Sep 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·S TauszigJ L Imler
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S Uttenweiler-JosephP Bulet
Nov 6, 2009·Journal of Biomedicine & Biotechnology·Xiao-Juan DengPeilin Xu
Dec 19, 2009·PLoS Pathogens·Mathilde GendrinBruno Lemaitre
Oct 19, 2001·Molecular Plant-microbe Interactions : MPMI·G J RairdanT P Delaney
Apr 20, 2004·Biochemical and Biophysical Research Communications·Evy VierstraeteLiliane Schoofs
Oct 10, 2006·Trends in Ecology & Evolution·Mara K N LawniczakTracey Chapman
Feb 16, 2018·The Journal of Experimental Biology·Sarah ChérasseJacobus J Boomsma
May 2, 2000·The Journal of Biological Chemistry·K Taniai, S Tomita
Sep 3, 2019·Frontiers in Immunology·Guillaume TetreauYannick Moret
May 26, 2017·Natural Product Reports·Michael GrafDaniel N Wilson
Oct 23, 1997·Biochemical and Biophysical Research Communications·S FurukawaM Yamakawa
Aug 1, 1997·Trends in Cell Biology·J A Hergannan, J V Rechhart

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