PMID: 11911590Mar 26, 2002Paper

Cloning of the genomes of equine herpesvirus type 1 (EHV-1) strains KyA and racL11 as bacterial artificial chromosomes (BAC)

Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health
Jens RudolphNikolaus Osterrieder

Abstract

The genome of equine herpesvirus type 1 (EHV-1) strain RacL11, a highly virulent isolate obtained from an aborted foal, and that of the modified live vaccine strain KyA, were cloned as bacterial artificial chromosomes (BAC) in Eseherichia coli. Mini F plasmid sequences were inserted into the viral genomes by homologous recombination instead of the gene 71 (EUS4) open reading frame after co-transfection of viral DNA and recombinant plasmid pdelta71-pHA2 into RK13 cells. After isolation of recombinant viruses by three rounds of plaque purification, viral DNA was isolated from RK13 cells infected with RacL11 or KyA virus mutants expressing the green fluorescent protein (GFP), and electroporated into Escherichia coli DH10B cells. Several bacterial colonies were shown to contain high-molecular weight BAC DNA with a restriction enzyme fragment pattern indicative of the presence of full-length RacL11 or KyA genomes. Two selected BAC clones were analysed by restriction enzyme analysis and Southern blotting, and were eventually termed pRacLI I and pKyA. respectively. Upon transfection of pRacL11 or pKyA DNA into RK13 cells, GFP-expressing fluorescing virus plaques could be identified from day 1 after transfection. Infectivity after tran...Continue Reading

References

Mar 1, 1996·The Journal of General Virology·Y SunS M Brown
Mar 1, 1996·Zentralblatt für Veterinärmedizin. Reihe A·A A HusseinK Schellander
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·M MesserleU H Koszinowski
Jun 25, 1998·Revue Scientifique Et Technique·N Osterrieder, E Wolf
Oct 15, 1998·Nature Genetics·Y ZhangA F Stewart
Feb 26, 1999·Nucleic Acids Research·J P MuyrersA F Stewart
May 29, 2000·Trends in Genetics : TIG·W BruneU H Koszinowski
Mar 27, 2001·Journal of Virology·H GranzowT C Mettenleiter

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Citations

Dec 9, 2003·Virus Research·Haijun YaoDennis J O'Callaghan
Jul 12, 2002·Trends in Microbiology·Markus WagnerUlrich H Koszinowski
Jan 29, 2008·Applied and Environmental Microbiology·Torsten HainTrinad Chakraborty
Sep 10, 2010·Journal of Virology·Helga Hofmann-SieberRalf Wagner
Mar 22, 2013·Journal of Virology·Walid AzabNikolaus Osterrieder
Apr 14, 2005·Journal of Virology·Sascha TrappNikolaus Osterrieder
Nov 5, 2010·Journal of Biomedicine & Biotechnology·Charles WardenHua Zhu
Apr 13, 2012·Journal of Biomedicine & Biotechnology·B Karsten Tischer, Benedikt B Kaufer
Feb 5, 2011·Veterinary Microbiology·Walid AzabHiroomi Akashi
Mar 5, 2010·The Journal of General Virology·Guanggang MaNikolaus Osterrieder
Feb 3, 2006·Expert Review of Vaccines·Cristina T RosasNikolaus Osterrieder
Jan 31, 2020·Journal of Virology·Jolien Van CleemputHans J Nauwynck

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