PMID: 9632573Jun 25, 1998Paper

Cloning of the heat shock protein 70 (HSP70) gene of Ehrlichia sennetsu and differential expression of HSP70 and HSP60 mRNA after temperature upshift

Infection and Immunity
Y ZhangY Rikihisa

Abstract

Ehrlichia sennetsu is the causative agent of human Sennetsu ehrlichiosis. Heat shock protein 60 (HSP60) and HSP70 (DnaK) are two major bacterial HSPs, and their interaction modulates the stress response. Previously, we cloned and sequenced groE and expressed groEL of E. sennetsu. HSP60 (GroEL) was immunogenic and cross-reactive in Ehrlichia spp. The present study was designed to (i) characterize the HSP70 gene of this organism and (ii) determine whether the expression of these two HSPs is inducible upon exposure to heat stress. A gene encoding an HSP70 homolog was isolated and sequenced from a gene library. The ehrlichial HSP70 gene encoded a 637-amino-acid protein, which had an approximate molecular mass of 68,354 Da and which was homologous to DnaK of Escherichia coli. A DNA sequence resembling -35 and -10 promoter sequences of E. coli dnaK was observed upstream of the ehrlichial HSP70 gene. Alignment of the predicted amino acid sequence with that of E. coli DnaK and Brucella, Salmonella, Borrelia, Chlamydia, and Mycobacterium HSP70s showed 63, 67, 63, 62, 58, and 53% identity, respectively. By reverse transcription-PCR analysis, the mRNA levels of ehrlichial HSP70 and HSP60 were examined after temperature shifts from 28 to 3...Continue Reading

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Citations

Nov 11, 1998·FEMS Microbiology Letters·M E EremeevaG A Dasch
Sep 27, 2008·Pakistan Journal of Biological Sciences : PJBS·N AmirmozafariR Rajabnia
Aug 15, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julio CarrionChristopher W Cutler
Feb 13, 2008·FEMS Microbiology Reviews·My-Van LaPatricia Renesto
Nov 26, 2019·Saudi Journal of Biological Sciences·Abdulaziz S AlqarniBrian H Smith

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