Cloning of the mucosal addressin MAdCAM-1 from human brain: identification of novel alternatively spliced transcripts

Immunology and Cell Biology
Euphemia LeungGeoffrey W Krissansen

Abstract

The mucosal addressin cell adhesion molecule-1 (MAdCAM-1), expressed selectively on high endothelial venules (HEV) and lamina propria venules, directs lymphocyte traffic by binding the lymphocyte Peyer's patch adhesion molecule-1 (LPAM-1, alpha 4 beta 7). Full-length DNA encoding human MAdCAM-1 was obtained by combining sequences from an expressed sequence tag (EST) identified in an early stage human brain cDNA library, a polymerase chain reaction-derived clone, and a MAdCAM-1 genomic clone. The deduced amino acid sequence revealed an 18 amino acid signal peptide, two N-terminal immunoglobulin (Ig)-like domains conserved (59-65%) in sequence with those of the mouse homologue, an 86 amino acid mucin-like region rich in serine-threonine residues, a 20 amino acid transmembrane domain and a 43 amino acid charged cytoplasmic domain. No counterpart to the third IgA-like domain of mouse MAdCAM-1 was present; however, the serine-threonine-rich mucin domain was extended as two distinguishable major and minor mucin regions unrelated to the mouse domain. The major domain is formed from six tandem repeats of an eight amino acid sequence having the MUC-2-related consensus DTTSPEP/SP. Human MAdCAM-1 mRNA transcripts were restricted to small ...Continue Reading

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Citations

Feb 17, 2001·Infection and Immunity·J U IgietsemeL L Perry
Jun 30, 2010·International Journal of Pediatric Endocrinology·Mei-Hong WenFuu-Jen Tsai
Mar 18, 2000·Leukemia & Lymphoma·S M Watt, J Y Chan
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Jul 31, 2004·Immunology and Cell Biology·Euphemia LeungGeoffrey W Krissansen
Mar 1, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Alice MilesDavid H Adams
Sep 24, 2020·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Zübeyde ÖztelErdal Balcan
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Jul 1, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Marisol VenyJulián Panés

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