PMID: 6413077Sep 1, 1983Paper

Cloning of the protective antigen gene of Bacillus anthracis

Cell
M Vodkin, S H Leppla

Abstract

The tripartite protein toxin of Bacillus anthracis consists of protective antigen (PA), edema factor (EF), and lethal factor (LF). As a first step in developing a more efficacious anthrax vaccine, recombinant plasmids containing the PA gene have been isolated. A library was constructed in the E. coli vector pBR322 from Bam HI-generated fragments of the anthrax plasmid, pBA1. Two clones producing PA were identified by screening lysates with ELISA (enzyme-linked immunosorbent assay). Western blots revealed a full-size PA protein in the recombinant E. coli, and a cell elongation assay demonstrated biological activity. Both positive clones had a 6 kb insert of DNA, which mapped in the Bam HI site of the vector. The two inserts are the same except that they lie in opposite orientations with respect to the vector. Thus PA is encoded by the plasmid pBA1.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1979·Transactions of the Royal Society of Tropical Medicine and Hygiene·M L McLarenJ E Lillywhite
Jan 25, 1983·Nucleic Acids Research·M YangD Henner
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·N VasanthaE Freese
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S H Leppla
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J M GonzálezB C Carlton
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H E Schnepf, H R Whiteley
May 1, 1963·Journal of General Microbiology·J L STANLEY, H SMITH
Nov 1, 1963·Proceedings of the Society for Experimental Biology and Medicine·D M MOLNAR, R A ALTENBERN
Apr 1, 1962·American Journal of Public Health and the Nation's Health·P S BrachmanN R Ingraham

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Citations

Mar 1, 1988·European Journal of Epidemiology·B E Ivins, S L Welkos
Jul 26, 2008·Applied Biochemistry and Biotechnology·Fatemeh VahediMahmoud Mahmoudi
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·D K LarsonJ W Burnett
Apr 2, 2004·Trends in Biochemical Sciences·Marcin Grynberg, Adam Godzik
Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·F Brossier, M Mock
Sep 29, 2001·Journal of Applied Microbiology·L Baillie
Oct 4, 1984·The New England Journal of Medicine·N C Engleberg, B I Eisenstein
Jan 1, 1984·Biotechnology & Genetic Engineering Reviews·M D Winther, G Dougan
Jan 16, 2013·Infection and Immunity·So-Young OhOlaf Schneewind
Mar 18, 2000·Infection and Immunity·F BrossierJ C Sirard
Dec 22, 2005·Infection and Immunity·Andrei P PomerantsevStephen H Leppla
May 23, 2012·Journal of Bacteriology·Sao-Mai Nguyen-MauOlaf Schneewind
Dec 14, 2001·Journal of Bacteriology·James PannucciCheryl R Kuske
Jan 2, 2004·Journal of Bacteriology·Melissa DrysdaleTheresa M Koehler
Mar 30, 2007·Journal of Clinical Microbiology·David SuePatricia P Wilkins
Aug 4, 2001·Biochemical and Biophysical Research Communications·N AhujaR Bhatnagar
Dec 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·J E Purser, S J Norris
Oct 31, 1991·Biochemical and Biophysical Research Communications·S F Little, J R Lowe
Mar 28, 2008·Molecular Microbiology·Justin W Kern, Olaf Schneewind
Nov 13, 2009·Molecular Microbiology·Justin Kern, Olaf Schneewind
Jan 1, 1988·Microbial Pathogenesis·S L Welkos, A M Friedlander
May 20, 2004·International Journal of Medical Microbiology : IJMM·Tâm MignotAgnès Fouet
Jun 7, 2005·Advanced Drug Delivery Reviews·Robert N Brey
Sep 10, 2005·Biochemical and Biophysical Research Communications·Shuchi MidhaRakesh Bhatnagar
Dec 16, 1987·Biochemical and Biophysical Research Communications·R L Kaspar, D L Robertson
Nov 1, 2006·Molecular Microbiology·Luciano A Marraffini, Olaf Schneewind
Mar 5, 2011·Molecular Microbiology·So-Young OhOlaf Schneewind
Jul 30, 1999·Protein Expression and Purification·P GuptaR Bhatnagar
Sep 7, 2001·Annual Review of Microbiology·M Mock, A Fouet

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