PMID: 9188599Jul 1, 1997Paper

Cloning of the rainbow trout (Oncorhynchus mykiss) Mx2 and Mx3 cDNAs and characterization of trout Mx protein expression in salmon cells

Journal of Virology
G D TrobridgeJ A Leong

Abstract

Two rainbow trout (Oncorhynchus mykiss) Mx cDNAs were cloned by using RACE (rapid amplification of cDNA ends) PCR and were designated RBTMx2 and RBTMx3. The deduced RBTMx2 and RBTMx3 proteins were 636 and 623 amino acids in length with molecular masses of 72 and 70.8 kDa, respectively. These proteins, along with the previously described RBTMx1 protein (G. D. Trobridge and J. A. Leong, J. Interferon Cytokine Res. 15:691-702, 1995), have between 88.7 and 96.6% identity at the amino acid level. All three proteins contain the tripartite GTP binding domain and leucine zipper motif common to Mx proteins. A monospecific polyclonal antiserum to an Escherichia coli-expressed fragment of RBTMx3 was generated, and that reagent was found to react with all three rainbow trout Mx proteins. Subsequently, endogenous Mx production in RTG-2 cells induced with poly(IC) double-stranded RNA was detected by immunoblot analysis. The cellular localization of the rainbow trout proteins was determined by transient expression of the RBTMx cDNAs in CHSE-214 (chinook salmon embryo) cells. A single-cell transient-transfection assay was used to examine the ability of each Mx cDNA clone to inhibit replication of the fish rhabdovirus infectious hematopoietic n...Continue Reading

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Citations

Jan 23, 1999·Immunological Reviews·J C LeongB Simon
Jan 10, 2003·The Journal of Veterinary Medical Science·Atsushi AsanoTomomasa Watanabe
Mar 16, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Teppei NakamuraTakashi Agui
Oct 22, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·N M EllinwoodR A Bowen
Aug 30, 2008·Immunogenetics·Sofia BerlinHans Ellegren
Jan 24, 1998·The Journal of Biological Chemistry·K Melén, I Julkunen
Jul 16, 2004·Journal of Virology·Rannveig LarsenBørre Robertsen
Oct 31, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Børre RobertsenArtur Albuquerque
Aug 24, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·V Jensen, B Robertsen
Feb 23, 2012·Viruses·Maureen K PurcellJames R Winton
Apr 26, 2020·Fish & Shellfish Immunology·Janet VelázquezYamila Carpio
Sep 20, 2000·Fish & Shellfish Immunology·R NygaardB Robertsen
Dec 16, 1997·Developmental and Comparative Immunology·B RobertsenJ A Leong
Oct 17, 2001·Developmental and Comparative Immunology·A E Ellis
Oct 17, 2001·Developmental and Comparative Immunology·B G Magor, K E Magor
Mar 19, 2011·Developmental and Comparative Immunology·Eloi R VerrierPierre Boudinot
Jul 9, 2004·Developmental and Comparative Immunology·Veronica Bergan, Børre Robertsen
Jun 9, 2005·Fish & Shellfish Immunology·Børre Robertsen
Jun 13, 2006·Fish & Shellfish Immunology·M Alejandra Fernández-TrujilloJulia Béjar
Aug 3, 2021·Virus Research·Mei HeCheng-Qiang He
Oct 31, 2006·Biochemical and Biophysical Research Communications·Chih-Hung LinChi-Yao Chang
Nov 6, 2007·Fish & Shellfish Immunology·Shona K Whyte
Apr 1, 2010·BMC Immunology·Astrid SkjesolJorunn B Jørgensen
Jan 29, 2008·Developmental and Comparative Immunology·Young-Mao ChenTzong-Yueh Chen

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Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
G D Trobridge, J A Leong
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