PMID: 375032Mar 20, 1979Paper

Cloning of the replication gene P of bacteriophage lambda: effects of increased P-protein synthesis on cellular and phage DNA replication

Molecular & General Genetics : MGG
J Klinkert, A Klein

Abstract

A restriction fragment of lambdaDNA carrying the P gene was cloned in the high copy number plasmid RSF2124. Cells harbouring this new plasmid RSF2124/lambdaE complement lambdaPam80 phage. A lac promoter-operator region (lacP), produced by EcoRI digestion of plasmid pKB252, was inserted into RSF2124/lambdaE such that induction of the lac promoter by IPTG or lactose leads to increased production of the P gene product. A high amount of P protein in E. coli cells results in a slow inhibition of bacterial DNA synthesis, suggesting that the initiation reaction is blocked by P protein. Synthesis of lambdaDNA proceeds normally under these conditions. Nonsuppressing groPA15 mutant bacteria which are unable to support the replication of wild-type lambda (lambdawt), acquire the ability to replicate lambdaPam80 phage but not lambdawt when they are transformed with a plasmid carrying the lambdaP gene. When harbouring a plasmid containing the mutant Pamber 80 gene, groPA15 mutants are able to support the replication of lambdawt phage when infected at a high multiplicity. lambdaPam80 phage does not multiply in these cells.

References

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Citations

Jan 1, 1981·Molecular & General Genetics : MGG·R A Sclafani, J A Wechsler
Jan 1, 1982·Molecular & General Genetics : MGG·T TsurimotoK Matsubara
Jan 1, 1981·Molecular & General Genetics : MGG·T Tsurimoto, K Matsubara
Jan 1, 1985·Folia Microbiologica·J Nesvera, J Hochmannová
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T Tsurimoto, K Matsubara
Oct 3, 2009·Journal of Molecular Biology·M AvlundS Krishna
Jan 1, 1981·Plasmid·K Matsubara

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