PMID: 8464695Mar 11, 1993Paper

Cloning of the zebrafish krox-20 gene (krx-20) and its expression during hindbrain development

Nucleic Acids Research
E Oxtoby, T Jowett

Abstract

To begin to examine the function of genes that control early development in the hindbrain, we have screened an embryonic zebrafish cDNA library with a murine krox-20 gene probe that contained the conserved zinc finger regions. We have isolated two overlapping cDNAs, zf187 and zf201 which are homologues of the murine krox-20 gene. The N-terminal of the longest cDNA (zf201) contains two acidic regions identical to those of the murine krox-20. This indicates that the functional organisation of these proteins is probably conserved. Northern Blot analysis identified a single transcript of 2.0 kb. Wholemount in situ hybridisation established that expression of the zebrafish gene (krx-20) first appears at 100% epiboly as a single anterior domain of the prospective neuroepithelium, followed very soon after by a second more posterior domain. The alternating pattern of expression of this gene in rhombomeres(r) r3 and r5 is apparent by 12 hr post-fertilisation, that is prior to the morphological appearance of the rhombomeres. Around 14 hr neural crest migration begins from the dorsal surface of r5, moving caudally into r6 and then ventrally towards the pharyngeal arches. Crest migration is not apparent at or after 16 hr. No neural crest m...Continue Reading

References

May 25, 1992·Nucleic Acids Research·C Vesque, P Charnay
Nov 1, 1991·PCR Methods and Applications·J B Lorens
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P CulpN Hopkins
Mar 7, 1991·Biochimica Et Biophysica Acta·J Garcia-BustosM N Hall
Aug 1, 1989·Trends in Genetics : TIG·C B Kimmel
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P LemaireP Charnay
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L J JosephV P Sukhatme
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B A ChristyD Nathans
Mar 1, 1988·Developmental Biology·D J GrunwaldG Streisinger
Aug 1, 1986·Developmental Biology·D M Noden
Oct 24, 1973·Nature: New Biology·A Garcia-BellidoG Morata

❮ Previous
Next ❯

Citations

Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y L YanJ H Postlethwait
Jul 1, 1994·Molecular Biology Reports·T Pieler, E Bellefroid
Jul 31, 2008·Cell and Tissue Research·Qin LiuAmy L Wilson
Feb 6, 2004·Brain Research. Developmental Brain Research·Nicole M Roy, Charles G Sagerström
Feb 20, 2003·Developmental Biology·Arne C LekvenRandall T Moon
Oct 1, 1996·Trends in Genetics : TIG·T Jowett, Y L Yan
Jun 14, 2002·Molecular and Cellular Endocrinology·E AyasoL Byrnes
Jun 12, 2012·Nature Cell Biology·Jingli CaoXueliang Zhu
Jan 15, 2008·Nature Protocols·Christine Thisse, Bernard Thisse
Jul 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Martin DistelReinhard W Köster
Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Zhenfei LiLin Li
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·S NonchevR Krumlauf
Jul 7, 2011·The Journal of Biological Chemistry·Ganesh V SamantRamani Ramchandran
Apr 30, 2011·The Journal of Biological Chemistry·Xiang ZhaoHeikki Rauvala
Aug 29, 2012·The Journal of Biological Chemistry·Rayk StengelChristoph Englert
Feb 6, 2008·Zebrafish·Evdokia DodouJohn H Postlethwait
Apr 21, 2009·Zebrafish·Adrian J HillRichard E Peterson
Apr 21, 2009·Zebrafish·Xu WangRichard I Dorsky
Oct 3, 2009·Human Molecular Genetics·Tanya L HenshallRobert I Richards
Aug 4, 2012·Human Molecular Genetics·Tamar E SztalPeter D Currie
Feb 19, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jedd M HillegassLori A White
Feb 26, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Lixin YangUwe Strähle
Jan 19, 2010·Genes & Development·Dorothy F SobieszczukDavid G Wilkinson
Dec 22, 2000·Genes & Development·E M GonzalezL Solnica-Krezel
Sep 1, 2007·Blood·Lisa J McReynoldsTodd Evans

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.