PMID: 9526500Apr 4, 1998Paper

Cloning of tobacco genes that elicit the hypersensitive response

Plant Molecular Biology
E E KarrerC A Holt

Abstract

We used a functional screening method to isolate genes whose products elicit the hypersensitive response (HR) pathway of defense against plant pathogens. A cDNA library derived from tobacco leaves undergoing the HR was cloned into a tobacco mosaic virus (TMV)-based expression vector. Infectious transcripts were generated and used to inoculate tobacco plants lacking the N resistance gene (genotype Xanthi nn). Approximately 1/1000 of the infectious transcripts produced local lesions, and may thus elicit the HR. The cDNA inserts from 50 lesion-forming clones were recovered by RT-PCR, and 12 unique clones were sequenced. Comparisons with protein databases revealed homologies to (a) ubiquitin, (b) tobacco tumor-related protein, similar to Kunitz-type trypsin inhibitors and (c) ribosomal protein S14. The remaining nine clones revealed no homology to known proteins and are thus considered novel. Five clones were able to induce the expression of PR2, a gene which is specifically activated in the tobacco HR. Northern and western blot analyses of leaves infected by the clone encoding ubiquitin strongly suggest that the infection produced a co-suppression response; the endogenous level of ubiquitin mRNA and protein in infected leaves are ...Continue Reading

Citations

Jun 13, 2002·Molecular Plant-microbe Interactions : MPMI·Anne VaretJustin Lee
Jul 15, 2006·Molecular Plant-microbe Interactions : MPMI·Alban JacquesSerge Kauffmann
Mar 5, 2015·Journal of Integrative Plant Biology·Liu YangQing Yang
May 8, 2018·Pest Management Science·Senthilvadivel VajravijayanKrishnasamy Gunasekaran
Sep 6, 2000·The Plant Journal : for Cell and Molecular Biology·I R ChoiR French
Nov 28, 2001·The Plant Journal : for Cell and Molecular Biology·G L BucherV A Iglesias
Aug 21, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·N RostoksA Kleinhofs
Mar 1, 2003·The Plant Journal : for Cell and Molecular Biology·Sang Sook LeeHyun-Sook Pai
Jan 11, 2003·Biotechnology & Genetic Engineering Reviews·Claudine Porta, George P Lomonossoff
Jul 16, 2011·The Journal of Biological Chemistry·Yiping QiFumiaki Katagiri
Apr 14, 2012·Plant Signaling & Behavior·Yiping Qi, Fumiaki Katagiri
Oct 20, 2017·Frontiers in Plant Science·Afsana IslamMichael T McManus
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·T H Turpen
Mar 3, 2010·Molecular Plant-microbe Interactions : MPMI·Michele PerazzolliClaudio Moser
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·F L TakkenM H Joosten
Dec 4, 2003·Molecular Plant-microbe Interactions : MPMI·Annalisa PolverariMassimo Delledonne
Mar 5, 2002·The Plant Journal : for Cell and Molecular Biology·Jack R PeartDavid C Baulcombe
Sep 4, 2012·Plant Molecular Biology·Koppolu Raja Rajesh Kumar, P B Kirti
Feb 21, 2016·Current Opinion in Plant Biology·Degao LiuXiaohan Yang
Aug 25, 2015·Plant Physiology·Toshiki IshikawaMaki Kawai-Yamada
Aug 11, 2000·Plant Science : an International Journal of Experimental Plant Biology·J WangJ H Oard
Aug 30, 2008·Phytochemistry·Alexander V KonarevPeter R Shewry

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