PMID: 9652399Jul 4, 1998Paper

Cloning of two cDNAs encoding three small serine protease inhibiting peptides from the desert locust Schistocerca gregaria and analysis of tissue-dependent and stage-dependent expression

European Journal of Biochemistry
Jozef Vanden BroeckArnold De Loof

Abstract

This study describes the cloning of two cDNAs encoding three serine-protease-inhibiting peptides, SGPI I, II and III, which were recently identified from ovarian extracts of the desert locust, Schistocerca gregaria. The first cDNA codes for the precursor polypeptides of SGPI I and SGPI II; the second encodes only a single inhibitor, SGPI III. Northern-blot analysis revealed an approximate length of 0.8 kb for SGPI-I/II mRNA and 0.6 kb for SGPI-III mRNA. The transcripts are present in several locust tissues, but they could not be detected in the midgut. The gene for SGPI-I/II is abundantly transcribed during all larval and adult stages, whereas SGPI-III mRNA is mainly present in adults. Northern-blot hybridization also revealed important changes in the SGPI-mRNA content during the molting cycle and during the adult reproductive cycle. Moreover, a differential hormonal control was observed in adult females which had been treated with precocene, juvenile hormone or ecdysone.

Citations

Jul 30, 1999·Developmental and Comparative Immunology·M R Kanost
May 30, 2001·Insect Biochemistry and Molecular Biology·A CherquiN Simões
May 9, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Gert SimonetJozef Vanden Broeck
May 14, 2009·BMC Evolutionary Biology·Bert BreugelmansJozef Vanden Broeck
Apr 15, 2009·Insect Biochemistry and Molecular Biology·Bert BreugelmansJozef Vanden Broeck
Jan 24, 2006·Biochemical and Biophysical Research Communications·Ilse ClaeysJozef Vanden Broeck
Apr 6, 2004·Biochemical and Biophysical Research Communications·Gert SimonetJozef Vanden Broeck
Aug 27, 2002·Fish & Shellfish Immunology·So Young Lee, Kenneth Söderhäll
Jun 20, 2006·Archives of Insect Biochemistry and Physiology·Ilse ClaeysJozef Vanden Broeck
Dec 17, 2008·General and Comparative Endocrinology·Heleen VerlindenJozef Vanden Broeck

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