Cloning of two individual cDNAS encoding 9-cis-epoxycarotenoid dioxygenase from Gentiana lutea, their tissue-specific expression and physiological effect in transgenic tobacco

Journal of Plant Physiology
Changfu ZhuGerhard Sandmann

Abstract

Two 9-cis-epoxycarotenoid dioxygenase (NCED) cDNAs have been cloned from a petal library of Gentiana lutea. Both cDNAs carry a putative transit sequence for chloroplast import and differ mainly in their length and the 5'-flanking regions. GlNCED1 was evolutionary closely related to Arabidopsis thaliana NCED6 whereas GlNCED2 showed highest homology to tomato NCED1 and A. thaliana NCED3. The amounts of GlNCED2 transcript were below Northern detection in G. lutea. In contrast, GlNCED1 was specifically expressed at higher levels in developing flowers when petals start appearing. By genetic engineering of tobacco with coding regions of either gene under a constitutive promoter, their function was further analyzed. Although mRNA of both genes was detectable in the corresponding transgenic plants, a physiological effect was only found for GlNCED1 but not for GlNCED2. In germination experiments of GlNCED1 transgenic lines, delayed radicle formation and cotyledon appearance were observed. However, the transformants exhibited no improved tolerance against desiccation stress. In contrast to other plants with over-expressed NCEDs, prolonged delay of seed germination is the only abscisic-acid-related phenotypic effect in the GlNCED1 transge...Continue Reading

References

Nov 26, 1984·Nucleic Acids Research·M Bevan
Jun 20, 1997·Science·S H SchwartzD R McCarty
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·B C TanD R McCarty
May 25, 1999·Protein Science : a Publication of the Protein Society·O EmanuelssonG von Heijne
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·X Qin, J A Zeevaart
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·A J ThompsonI B Taylor
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C D Rock, J A Zeevaart
Apr 15, 2003·Plant Physiology·Steven H SchwartzJan A D Zeevaart
Jul 2, 2003·The Plant Journal : for Cell and Molecular Biology·Bao-Cai TanDonald R McCarty
Mar 9, 2005·Current Opinion in Plant Biology·Frank GublerJohn V Jacobsen
May 3, 2005·Annual Review of Plant Biology·Eiji Nambara, Annie Marion-Poll

❮ Previous
Next ❯

Citations

Jul 12, 2014·Journal of Plant Physiology·Akira EndoYutaka Sato
Aug 2, 2011·Plant Science : an International Journal of Experimental Plant Biology·Ikuo AshikawaShingo Nakamura
Oct 31, 2009·Plant Signaling & Behavior·María del Carmen Rodríguez-GacioAngel J Matilla
Jan 1, 2010·Biotechnology & Genetic Engineering Reviews·Carlo RosatiGiovanni Giuliano
Aug 7, 2010·Archives of Biochemistry and Biophysics·Changfu ZhuPaul Christou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.