PMID: 8606192Apr 1, 1996Paper

Cloning of type 8 capsule genes and analysis of gene clusters for the production of different capsular polysaccharides in Staphylococcus aureus

Journal of Bacteriology
S Sau, C Y Lee

Abstract

Eleven serotypes of capsular polysaccharide from Staphylococcus aureus have been reported. We have previously cloned a cluster of type 1 capsule (cap1) genes responsible for type 1 capsular polysaccharide biosynthesis in S. aureus M. To clone the type 8 capsule (cap8) genes, a plasmid library of type 8 strain Becker was screened with a labelled DNA fragment containing the cap1 genes under low-stringency conditions. One recombinant plasmid containing a 14-kb insert was chosen for further study and found to complement 14 of the 18 type 8 capsule-negative (Cap8-) mutants used in the study. Additional library screening, subcloning, and complementation experiments showed that all of the 18 Cap8- mutants were complemented by DNA fragments derived from a 20.5-kb contiguous region of the Becker chromosome. The mutants were mapped into six complementation groups, indicating that the cap8 genes are clustered. By Southern hybridization analyses under high-stringency conditions, we found that DNA fragments containing the cap8 gene cluster show extensive homology with all 17 strains tested, including type 1 strains. By further Southern analyses and cloning of the cap8-related homolog from strain M, we show that strain M carries an additiona...Continue Reading

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Citations

Apr 28, 2000·Microbiology·P M PowerM P Jennings
Aug 24, 2000·The Journal of Biological Chemistry·C CreuzenetJ S Lam
Nov 24, 2007·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·S C Deresinski
Jan 20, 2006·Molecular Microbiology·Jordan L CocchiaroJean C Lee
Jan 27, 1999·FEMS Microbiology Letters·E R WannT J Foster
May 11, 2002·The Journal of Biological Chemistry·Carole CreuzenetJoseph S Lam
Oct 16, 1999·Glycobiology·D Kapitonov, R K Yu
Jun 18, 2002·Infection and Immunity·Thanh T Luong, Chia Y Lee
Jun 19, 2003·Journal of Bacteriology·Thanh T LuongChia Y Lee
Sep 8, 1999·Microbiology and Molecular Biology Reviews : MMBR·H L RocchettaJ S Lam
Jun 9, 2004·Journal of Clinical Microbiology·Markus HufnagelJohannes Huebner

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