Cloning of xcp genes located at the 55 min region of the chromosome and involved in protein secretion in Pseudomonas aeruginosa

Molecular Microbiology
A FillouxA Lazdunski

Abstract

Pleiotropic mutations (xcp) affecting secretion of proteins in Pseudomonas aeruginosa have been previously characterized and mapped at 0 min, 55 min and 65 min. Genomic libraries of this organism have been constructed and the genes xcp-5 and xcp-54, located at the 55 min region, were cloned using the adjacent met allele as a marker, and complementation of xcp strains. From our linkage and cloning analysis, the most probable gene order in this region appears to be pyrD... xcp-5/xcp-54/met-9011/oru-314/trpF/leu-10. Restriction mapping and transposon (Tn1725) insertion mutagenesis demonstrated that: (i) the overall size of DNA necessary for xcp expression was 9kb, (ii) the two loci are not adjacent on the chromosome, and (iii) the two loci are expressed independently. The xcp-5 gene has been subcloned on a 4kb EcoRI fragment.

References

Jul 20, 1977·Molecular & General Genetics : MGG·P M BennettM H Richmond
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Grunstein, D S Hogness
Nov 1, 1974·The Journal of Infectious Diseases·P V Liu
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Dec 1, 1981·Infection and Immunity·R M BerkaM L Vasil

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Citations

Aug 1, 1993·Enzyme and Microbial Technology·E J Gilbert
Feb 1, 1990·Biochimie·A LazdunskiM Murgier
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D N Nunn, S Lory
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D N Nunn, S Lory
Mar 14, 2007·Journal of Bacteriology·Gérard P F MichelAlain Filloux
Jul 28, 2012·Research in Microbiology·Geneviève BallAlain Filloux
Sep 1, 1992·FEMS Microbiology Reviews·J TommassenA Lazdunski
Sep 20, 2005·FEMS Microbiology Letters·Viviane RobertGérard P F Michel
Jan 1, 1996·Critical Reviews in Biotechnology·A EndrizziJ M Belin
Sep 1, 2010·EcoSal Plus·Marcella PatrickTanya L Johnson

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