PMID: 11341932May 9, 2001Paper

Cloning, purification and characterisation of Staphylococcus warneri lipase 2

Biochimica Et Biophysica Acta
M D van KampenM R Egmond

Abstract

A gene encoding an extracellular lipase was identified in Staphylococcus warneri 863. The deduced lipase is organised as a prepro-protein and has significant similarity to other staphylococcal lipases. The mature part of the lipase was expressed with an N-terminal histidine tag in Escherichia coli, purified and biochemically characterised. The results show that the purified lipase (named SWL2) combines the properties of the staphylococcal lipases characterised so far. It has both a high preference for short chain substrates and surprisingly, it also displays phospholipase activity. Homology alignment was used to analyse sequence-function relationships of the staphylococcal lipase family with the aim to identify the structural basis underlying the different properties of the staphylococcal lipases.

References

Nov 28, 1989·Biochemistry·M G van OortF Götz
Apr 1, 1994·Protein Engineering·M LottiL Alberghina
Feb 1, 1993·Journal of General Microbiology·A M FarrellK T Holland
Aug 28, 1998·Chemistry and Physics of Lipids·M D van KampenH M Verheij
Jul 22, 1999·Biochemistry·M D van KampenM R Egmond

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Citations

Oct 31, 2009·Molecular Biotechnology·Fábio Cristiano Angonesi BrodAna Carolina Maisonnave Arisi
Jul 27, 2010·Interdisciplinary Sciences, Computational Life Sciences·Saravanan ParameswaranSanjukta Patra
Mar 22, 2013·Genome Announcements·Victor W T ChengJoel H Weiner
Jul 28, 2015·Molecular Membrane Biology·Samuel FurseJ Antoinette Killian
Jun 22, 2007·Journal of Molecular Biology·Jan J W TiesingaBauke W Dijkstra
Nov 6, 2009·International Journal of Medical Microbiology : IJMM·Silva HoltfreterBarbara M Bröker
May 31, 2016·Journal of Biotechnology·Yu Jung KimSeung-Goo Lee
Apr 20, 2005·Biochimica Et Biophysica Acta·Habib MosbahYoussef Gargouri
Jul 27, 2012·Journal of Molecular Microbiology and Biotechnology·Ken-ji YokoiKen-Ichi Kodaira
Dec 1, 2006·Dermatology : International Journal for Clinical and Investigative Dermatology·Karin SartoriusMaria Hedberg
Jun 17, 2011·Molecular Biology Reports·Pushpender Kumar SharmaJagdeep Kaur
Apr 30, 2014·The Protein Journal·Nor Hafizah Ahmad KamarudinAbu Bakar Salleh

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