Cloning, purification, crystallization and preliminary crystallographic analysis of a hypothetical acetyltransferase from Pyrococcus furiosus

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Sabrina Biarrotte-Sorin, Claudine Mayer

Abstract

The GCN5-related N-acetyltransferase (GNAT) superfamily has a primordial role in cellular processes such as transcription initiation and regulation by histone acetylation, aminoglycoside resistance and melatonin metabolism. To date, no acetyltransferase from the archaeal domain of life has been studied. This paper describes the cloning, expression, purification and crystallization of a Pyrococcus furiosus hypothetical acetyltransferase PfGNAT (MW = 22 007 Da). The crystals belong to space group P622, with one molecule in the asymmetric unit and unit-cell parameters a = b = 82.6, c = 105.92 A, alpha = beta = 90, gamma = 120 degrees. Crystals diffract X-rays to 3.0 A resolution on a synchrotron-radiation source. Determination of this structure will provide new insights into the substrate-specificity of this acetyltransferase and the thermal stability of the N-acetyltransferase domain.

References

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Citations

Oct 5, 2010·Archaea : an International Microbiological Journal·Jörg Soppa
Oct 28, 2020·Journal of Pineal Research·Jean A Boutin, Ralf Jockers

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