Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of XC847, a 3'-5' oligoribonuclease from Xanthomonas campestris

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Yan-You WuShan-Ho Chou

Abstract

Oligoribonucleases are essential components of RNA and DNA metabolism and close homologues of genes encoding them are found not only in prokaryotes but also in a wide range of eukaryotes, including yeast and humans. Inactivation of the oligoribonuclease gene (orn) can result in cellular lethality. Despite their important biological function, they have been studied little from a structural point of view. In this report, the cloning, expression, crystallization and preliminary X-ray analysis of XC847, a DEDDh-type 3'-5' oligoribonuclease from the plant pathogen Xanthomonas campestris pv. campestris, a Gram-negative bacterium causing major worldwide disease of cruciferous crops, is described. The XC847 crystals diffracted to a resolution of at least 2.1 A. They are tetragonal and belong to space group P4(3)2(1)2, with unit-cell parameters a = b = 67.5, c = 89.8 A. One molecule is present per asymmetric unit.

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Citations

Oct 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Ying-Der TsaiShan-Ho Chou
Oct 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Kuo-Cheng ChioShan-Ho Chou
Nov 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Chien-Hung LinShan-Ho Chou
Dec 5, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Wen-Ting LoShan-Ho Chou
Dec 22, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sz-Kai RuanShan-Ho Chou
Jun 15, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jhe-Le TuShan-Ho Chou
Oct 24, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Xian Ya LinShan Ho Chou
Mar 24, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Yi-Ting LiaoShan-Ho Chou
Jul 16, 2011·PloS One·Tso-Ning LiShan-Ho Chou
Feb 14, 2009·Progress in Molecular Biology and Translational Science·José M AndradeCecília M Arraiano
Mar 7, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Jauo-Guey YangShan-Ho Chou
Sep 21, 2012·Acta Crystallographica. Section D, Biological Crystallography·Ko-Hsin ChinShan-Ho Chou
Oct 3, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Yu-Chuan WangShan-Ho Chou
Aug 8, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Yi-Che SuShan-Ho Chou

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