Cloning, purification, crystallization and preliminary crystallographic analysis of a penicillin-binding protein homologue from Pyrococcus abyssi

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Vanessa DelfosseClaudine Mayer

Abstract

The genome of the hyperthermophilic archaeon Pyrococcus abyssi contains a gene (pab0087) encoding a penicillin-binding protein (PBP) homologue. This sequence consists of 447 residues and shows significant sequence similarity to low-molecular-weight PBPs and class C beta-lactamases. The Pab0087 protein was overexpressed, purified and crystallized. Diffraction data from two different crystal forms were collected to 2.7 and 2.0 A resolution. Both crystals belong to space group C2, with unit-cell parameters a = 160.59, b = 135.74, c = 113.02 A, beta = 117.36 degrees and a = 166.97, b = 131.25, c = 189.39 A, beta = 113.81 degrees , respectively. The asymmetric unit contains four and eight molecules, respectively, with fourfold non-crystallographic symmetry.

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