Cloning, purification, crystallization and preliminary X-ray studies of a carbohydrate-binding module from family 64 (StX)

Acta Crystallographica. Section F, Structural Biology Communications
Bruna Medeia CamposFabio Marcio Squina

Abstract

In recent years, biofuels have attracted great interest as a source of renewable energy owing to the growing global demand for energy, the dependence on fossil fuels, limited natural resources and environmental pollution. However, the cost-effective production of biofuels from plant biomass is still a challenge. In this context, the study of carbohydrate-binding modules (CBMs), which are involved in guiding the catalytic domains of glycoside hydrolases to polysaccharides, is crucial for enzyme development. Aiming at the structural and functional characterization of novel CBMs involved in plant polysaccharide deconstruction, an analysis of the CAZy database was performed and CBM family 64 was chosen owing to its capacity to bind with high specificity to microcrystalline cellulose and to the fact that is found in thermophilic microorganisms. In this communication, the CBM-encoding module named StX was expressed, purified and crystallized, and X-ray diffraction data were collected from native and derivatized crystals to 1.8 and 2.0 Å resolution, respectively. The crystals, which were obtained by the hanging-drop vapour-diffusion method, belonged to space group P3121, with unit-cell parameters a = b = 43.42, c = 100.96 Å for the na...Continue Reading

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Sep 19, 2001·The Journal of Biological Chemistry·A C FreeloveH J Gilbert
Mar 9, 2004·The Journal of Biological Chemistry·David N BolamHarry J Gilbert
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Philip Evans
Jan 28, 2006·Science·Alexander E FarrellDaniel M Kammen
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Dec 20, 2008·Journal of Synchrotron Radiation·Beatriz G GuimarãesIgor Polikarpov
May 26, 2009·Acta Crystallographica. Section D, Biological Crystallography·Thomas C TerwilligerLi Wei Hung
Nov 13, 2009·Applied Microbiology and Biotechnology·Daniel GuillénRomina Rodríguez-Sanoja
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·T Geoff G BattyeAndrew G W Leslie
Jun 21, 2011·Applied and Environmental Microbiology·Angel AngelovWolfgang Liebl
Sep 17, 2011·Angewandte Chemie·Tae-Wan KimDouglas S Clark
Oct 12, 2012·Applied Microbiology and Biotechnology·In Jung KimKyoung Heon Kim
Jul 4, 2013·Biotechnology for Biofuels·Vimalier Reyes-OrtizDanielle Tullman-Ercek
Dec 21, 2013·PloS One·Thabata M AlvarezMario T Murakami
Sep 10, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Bruna Medeia CamposFabio Marcio Squina

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