PMID: 2106670Feb 11, 1990Paper

Cloning regions of the Drosophila genome by microdissection of polytene chromosome DNA and PCR with nonspecific primer

Nucleic Acids Research
C S WesleyW F Eanes

Abstract

A simple and rapid procedure to isolate clones carrying sequences from a specific region of the polytene chromosome of Drosophila is demonstrated. The procedure involves microdissection of the region of interest, amplification of the DNA by PCR using a primer designed to prime the synthesis nonspecifically, labeling of the amplified DNA using the random primer method, and screening of a standard library with the probe to identify and isolate clones carrying sequences homologous to the dissected region. This procedure has the potential to replace the difficult procedure of microcloning, as well as facilitate chromosome walking.

References

Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P R LangerD C Ward
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein

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Citations

May 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R D Saunders
Sep 1, 1991·Cancer Genetics and Cytogenetics·L A Cannizzaro
Jul 18, 2000·Journal of Agricultural and Food Chemistry·M L FauconnierM Paquot
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C S Wesley, W F Eanes
Dec 25, 1990·Nucleic Acids Research·J Welsh, M McClelland
Feb 11, 1994·Nucleic Acids Research·C S WesleyM W Young
Aug 1, 1995·Insect Molecular Biology·S E BrownD L Knudson
May 8, 2007·Applied and Environmental Microbiology·Shiming WangShunpeng Li
Aug 1, 1995·Genome Génome / Conseil National De Recherches Canada·Q Chen, K Armstrong
May 10, 2008·Genome Génome / Conseil National De Recherches Canada·R ZimmerA M Gibbins
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·A K BejR M Atlas
May 7, 2014·Small·Brandon K ChenDavid P Bazett-Jones

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