Cloning, sequence analysis, and expression of the major capsid protein of the iridovirus frog virus 3

Virology
J MaoV G Chinchar

Abstract

The nucleotide sequence of the gene encoding in the major capsid protein (MCP) of frog virus 3 (FV3) has been determined and compared to other iridovirus capsid genes. Nucleotide sequence and S1 nuclease analysis showed that the FV3 MCP gene encoded a transcript of 1452 nucleotides containing a 12 nucleotide AU-rich 5' nontranslated region (NTR) and a 50-nucleotide 3' NTR whose terminus was predicted to fold into a hairpin of moderate stability. An open reading frame initiating from the the 5'-most AUG codon encoded a protein of 463 amino acids with a predicted molecular weight of 49,860. Expression of the putative FV3 MCP gene in vitro confirmed that it encoded the major capsid protein of FV3 and supported the suggestion that translation initiated at AUG-1. Pairwise amino acid alignments detected a high degree of sequence identity between the FV3 MCP and other iridoviruses. These results indicate that iridoviruses possess an evolutionarily related major capsid protein and provide information useful not only in studies of viral gene expression, but also in characterizing newly isolated iridoviruses.

Citations

Jun 15, 2004·Journal of Virology·Qi-Ya ZhangJian-Fang Gui
Mar 1, 2005·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Ryan De VoeJames Guy
Dec 14, 2011·Viruses·Debra MillerAndrew Storfer
Feb 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Karen R LipsJames P Collins
Jun 27, 2007·ILAR Journal·Christine L Densmore, David Earl Green
May 14, 2005·Oecologia·Jesse L BrunnerJames P Collins
Nov 23, 2010·Journal of Virological Methods·Riikka HolopainenHannele Tapiovaara
May 8, 2015·In Vitro Cellular & Developmental Biology. Animal·P H PhamN C Bols
Jan 7, 2010·Journal of Fish Diseases·R J WhittingtonM M Dennis
Jul 26, 2011·Virology·Jacques RobertGuangchun Chen
Dec 3, 2014·Current Biology : CB·Stephen J PriceJaime Bosch
Nov 5, 1999·Annual Review of Microbiology·J L Van Etten, R H Meints
Feb 1, 2005·Virology·Jacques RobertJennifer Gantress
Sep 6, 2011·Virus Research·Li-Bo HeQi-Ya Zhang
Jan 2, 2018·Virusdisease·P SivasankarM J Prince Jeyaseelan
Sep 22, 2018·Veterinary Pathology·Marcos Isidoro-AyzaAurélie Chambouvet
Dec 17, 2011·EcoHealth·Jason T HovermanNathan A Haislip
Sep 2, 2017·Scientific Reports·Marcos Isidoro-AyzaWilliam J Barichivich
Feb 26, 2016·Diseases of Aquatic Organisms·Betsie B RothermelMichael J Yabsley
Jul 29, 1998·Journal of Molecular Biology·R TumaP E Prevelige

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