PMID: 9173871Apr 1, 1997Paper

Cloning, sequencing and characterization of the 5'-flanking region of the human collagenase-3 gene

The Biochemical Journal
G TardifJ Martel-Pelletier

Abstract

Collagenase-3 (matrix metalloprotease-13) is a recently discovered human collagenase produced in normal articular cartilage chondrocytes and thought to be involved in the pathological process of osteoarthritis. We have sequenced and characterized 1.6 kb of the human collagenase-3 gene 5'-flanking region. The transcription start site was located 22 bp upstream from the ATG start codon. Sequence analysis of the 5'-flanking region revealed the presence of the consensus recognition sites for the TATA and CCAAT DNA-binding proteins, activator protein-1 and E26 transformation specific/polyoma virus enhancer, as well as three core motifs of hormone response elements. Transient transfection assays demonstrated that a small fragment of 133 bp, containing the activator protein-1 and E26 transformation specific/polyoma virus enhancer sites promoted transcription in normal and osteoarthritic human chondrocytes with significantly higher activity than the original 1.6 kb fragment. Nucleotide sequence comparison of the promoter region of human collagenase-3 revealed a stronger similarity to the mouse collagenase-1 promoter than to the human collagenase-1 promoter.

Citations

Dec 2, 2009·BMC Musculoskeletal Disorders·Ginette TardifJohanne Martel-Pelletier
Sep 14, 1999·The American Journal of Physiology·R C PereiraE Canalis
Feb 6, 2013·PloS One·María C de AndrésRichard O C Oreffo
Dec 17, 2014·BMC Musculoskeletal Disorders·Karl AlvarezRichard O C Oreffo
Mar 27, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ching-Hao LiJaw-Jou Kang
Apr 23, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M InadaT Komori
Mar 9, 2002·Biochemical and Biophysical Research Communications·Mohamed BenderdourJohanne Martel-Pelletier
Feb 20, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Nanjun WuJeffrey M Davidson
Sep 8, 1998·Current Opinion in Genetics & Development·T Komori, T Kishimoto
Jul 28, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Carlos RossaKeith L Kirkwood

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