Cloning, sequencing and characterization of the gene encoding a principal sigma factor homolog from the cyanobacterium Microcystis aeruginosa K-81

Gene
Munehiko AsayamaMakoto Shirai

Abstract

We cloned and sequenced the rpoD1 gene of Microcystis aeruginosa K-81, a unicellular colony-forming cyanobacterium that can perform photosynthesis involving light-responsive gene expression. The deduced amino acid sequence of RpoD1 exhibited extensive homology to the other eubacterial principal sigma factors. Primer extension and Western blot analyses revealed that the rpoD1 gene, which encodes a principle sigma factor homolog, had two transcription start points, P1 and P2. These transcripts, and the corresponding protein, constitutively appeared in M. aeruginosa, irrespective of light or dark conditions.

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Citations

Mar 28, 2001·The Journal of Gene Medicine·M K ChuahT VandenDriessche
Sep 11, 2002·The Journal of Gene Medicine·Heshan Sam ZhouArthur L Beaudet
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Oct 20, 2009·Gene Regulation and Systems Biology·Sousuke Imamura, Munehiko Asayama

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