PMID: 9422599Jan 9, 1998Paper

Cloning, sequencing, and disruption of the Bacillus subtilis psd gene coding for phosphatidylserine decarboxylase

Journal of Bacteriology
K MatsumotoI Shibuya

Abstract

The psd gene of Bacillus subtilis Marburg, encoding phosphatidylserine decarboxylase, has been cloned and sequenced. It encodes a polypeptide of 263 amino acid residues (deduced molecular weight of 29,689) and is located just downstream of pss, the structural gene for phosphatidylserine synthase that catalyzes the preceding reaction in phosphatidylethanolamine synthesis (M. Okada, H. Matsuzaki, I. Shibuya, and K. Matsumoto, J. Bacteriol. 176:7456-7461, 1994). Introduction of a plasmid containing the psd gene into temperature-sensitive Escherichia coli psd-2 mutant cells allowed growth at otherwise restrictive temperature. Phosphatidylserine was not detected in the psd-2 mutant cells harboring the plasmid; it accumulated in the mutant up to 29% of the total phospholipids without the plasmid. An enzyme activity that catalyzes decarboxylation of 14C-labeled phosphatidylserine to form phosphatidylethanolamine was detected in E. coli psd-2 cells harboring a Bacillus psd plasmid. E. coli cells harboring the psd plasmid, the expression of which was under the control of the T7phi10 promoter, produced proteins of 32 and 29 kDa upon induction. A pulse-labeling experiment suggested that the 32-kDa protein is the primary translation produc...Continue Reading

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Citations

Aug 24, 2006·Molecular Microbiology·Kouji MatsumotoHiroshi Hara
Jul 29, 2003·The Journal of Biological Chemistry·Tim H SzetoGlenn F King
Feb 20, 2004·Journal of Bacteriology·Fumitaka KawaiKouji Matsumoto
Jan 24, 2009·IUBMB Life·Irmgard Schuiki, Günther Daum
Dec 16, 2010·The Journal of Biological Chemistry·Changrong GeAmélie A Kelly
Apr 12, 2015·FEMS Microbiology Reviews·Christian Sohlenkamp, Otto Geiger
Oct 7, 2003·Annual Review of Microbiology·John E Cronan
Jan 20, 2018·The Journal of Antibiotics·Reed M StubbendieckPaul D Straight
Nov 12, 2020·Fungal Genetics and Biology : FG & B·Lin TangHansong Dong
Apr 13, 2021·Frontiers in Molecular Biosciences·William Dowhan, Mikhail Bogdanov
Aug 26, 2009·Progress in Lipid Research·Otto GeigerChristian Sohlenkamp
May 22, 2007·Progress in Lipid Research·Athanasios Lykidis
Feb 22, 2011·Cell·Gyanendra P Dubey, Sigal Ben-Yehuda

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