Cloning, sequencing and expression analysis of the equine hepcidin gene by real-time PCR

Veterinary Immunology and Immunopathology
José P Oliveira FilhoAlexandre S Borges

Abstract

Equine serum or plasma iron concentration drops quickly during inflammation. Accumulation of iron inside macrophages and reduction of the intestinal absorption of this element cause hypoferremia during systemic inflammatory processes. These mechanisms are mediated by hepcidin, a 25 amino acids peptide synthesized mainly in the liver in response to iron stores and inflammation. Hepcidin is an important peptide for systemic iron homeostasis and also has antibacterial and antifungal activities. Hepcidin up-regulation is particularly useful during acute inflammation, especially before adaptive immunity occurs, restricting iron availability necessary for pathogenic microorganism growth. Hepcidin gene products have been previously characterized in man, non-human primates, rat, mouse, dog swine, cattle, fishes, reptiles and birds; but until now not in the horse. We have cloned and sequenced equine hepcidin mRNA and performed hepcidin expression analysis in different tissues collected from four healthy horses. The deduced precursor of equine hepcidin was most homologous to Bos taurus and Sus scrofa. The expressed profile of equine hepcidin in liver was very high. Expression in cervical spinal cord and cerebral cortex was much lower tha...Continue Reading

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Dec 17, 2010·Veterinary Research Communications·Victoria Chanu Khangembam, Ashok Kumar
Jan 31, 2012·The Veterinary Clinics of North America. Small Animal Practice·Carolyn N GrimesMichael M Fry
Aug 21, 2010·Research in Veterinary Science·Peres R BadialAlexandre S Borges
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Jun 19, 2013·The Veterinary Journal·Ana Claudia GorinoAlexandre S Borges
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Sep 20, 2011·Innate Immunity·José P Oliveira-FilhoAlexandre S Borges
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Mar 31, 2018·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Juliana MiraJosé P Oliveira-Filho
May 26, 2020·Reproduction in Domestic Animals = Zuchthygiene·Kandasamy RajamanickamAngappan Mangala Gowri
May 26, 2021·Peptides·C E Valdez-MiramontesB Rivas-Santiago

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