Cloning, sequencing, and expression of a gene from Campylobacter jejuni encoding a protein (Omp18) with similarity to peptidoglycan-associated lipoproteins.

Infection and Immunity
M E KonkelW Cieplak

Abstract

A Campylobacter jejuni genomic plasmid library was screened with antiserum generated against whole C. jejuni, revealing two immunoreactive clones. Sequence analysis of the recombinant plasmids revealed a common open reading frame of 498 nucleotides encoding a protein of 165 amino acids with a calculated molecular mass of 18,018 Da. The recombinant product partitioned to the outer membrane fractions of Escherichia coli transformants and has been designated Omp18. The deduced amino acid sequence of the cloned C. jejuni gene exhibits considerable similarity to peptidoglycan-associated lipoproteins from other gram-negative bacteria.

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Citations

Mar 1, 2016·Frontiers in Microbiology·Patrycja A KobiereckaAgnieszka K Wyszyńska
Mar 25, 2000·FEMS Microbiology Letters·D P PawelecE K Jagusztyn-Krynicka
Jun 24, 2003·Infection and Immunity·Petra VolandChristian Prinz
Dec 14, 2011·Clinical and Vaccine Immunology : CVI·Lene N NielsenKaren A Krogfelt
Nov 19, 2011·FEMS Immunology and Medical Microbiology·Zsuzsanna KovachHazel Mitchell

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