PMID: 2507528Oct 1, 1989Paper

Cloning, sequencing, and expression of the gene for NADH-sensitive citrate synthase of Pseudomonas aeruginosa

Journal of Bacteriology
L J DonaldH W Duckworth

Abstract

The structural gene for the allosteric citrate synthase of Pseudomonas aeruginosa has been cloned from a genomic library by using the Escherichia coli citrate synthase gene as a hybridization probe under conditions of reduced stringency. Subcloning of portions of the original 10-kilobase-pair (kbp) clone led to isolation of the structural gene, with its promoter, within a 2,083-bp length of DNA flanked by sites for KpnI and BamHI. The nucleotide sequence of this fragment is presented; the inferred amino acid sequence was 70 and 76% identical, respectively, with the citrate synthase sequences from E. coli and Acinetobacter anitratum, two other gram-negative bacteria. DEAE-cellulose chromatography of P. aeruginosa citrate synthase from an E. coli host harboring the cloned P. aeruginosa gene gave three peaks of activity. All three enzyme peaks had subunit molecular weights of 48,000; the proteins were identical by immunological criteria and very similar in kinetics of substrate saturation and NADH inhibition. Because the cloned gene contained only one open reading frame large enough to encode a polypeptide of such a size, the three peaks must represent different forms of the same protein. A portion of the cloned P. aeruginosa gene...Continue Reading

References

Jun 19, 1984·Biochemistry·V Bhayana, H W Duckworth

❮ Previous
Next ❯

Citations

May 15, 1993·European Journal of Biochemistry·A J PattonM J Danson
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·L Herrera-Estrella
Jan 1, 1993·Antonie van Leeuwenhoek·T Beppu
Mar 26, 1999·European Journal of Biochemistry·S M PitsonS L Hazell
Feb 29, 2008·Plant Cell Reports·Pierluigi BaroneWayne Parrott
Sep 1, 2005·Applied Microbiology and Biotechnology·Christian EweringAlexander Steinbüchel
Jan 1, 1992·Applied and Environmental Microbiology·F J SchendelM C Flickinger
Apr 1, 1990·Journal of Bacteriology·M FukayaT Beppu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Acinetobacter Infections

Acinetobacter infections have become common in hospitalized patients, especially in the intensive care unit setting and are difficult to treat due to their propensity to develop antimicrobial drug resistance. Discover the latest research on Acinetobacter Infections here.

© 2022 Meta ULC. All rights reserved