PMID: 12753502May 20, 2003Paper

Cloning, sequencing and expression of immunoglobulin variable regions of murine monoclonal antibodies specific for the P1.7 and P1.16 PorA protein loops of Neisseria meningitidis

Scandinavian Journal of Immunology
Øistein IhleT E Michaelsen

Abstract

The P1.7 and P1.16 epitopes on the PorA protein on the outer membrane of Neisseria meningitidis can induce protective antibodies upon vaccination. Structural analysis of antibodies to these targets can give information on the immune response induced by these epitopes and can reveal any structural similarities among the antibodies. To do so, we have isolated the immunoglobulin (Ig) variable genes from four mouse hybridomas expressing antibodies against the P1.7 and P1.16 epitopes. These V genes were successfully expressed as functional chimeric (ch) mouse/human IgG1 antibodies by subcloning them into expression vectors containing the constant genes of human heavy and light chains. Sequencing the two sets of V genes against P1.16 revealed a high degree of homology, similar to that previously published for P1.7 V genes. The close homology allowed us to interchange heavy and light chains between antibodies in some instances to construct new antibodies that bind the original antigen. This study demonstrates that the immune response in mice against the meningococcal PorA protein epitopes P1.7 as well as P1.16 is limited to few and very similar germline genes, and therefore the P1.7- and P1.16-specific antibodies share high degree of ...Continue Reading

References

Sep 1, 1991·Infection and Immunity·P van der LeyJ T Poolman
Dec 21, 1989·Nature·C ChothiaW R Tulip
Aug 10, 2000·Nucleic Acids Research·O Ihle, T E Michaelsen
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·G VidarssonJ G van De Winkel
Aug 3, 2001·Molecular Immunology·B B HainesP H Brodeur

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