PMID: 9642197Jun 27, 1998Paper

Cloning, sequencing, and phenotypic characterization of the rpoS gene from Pseudomonas putida KT2440

Journal of Bacteriology
M I Ramos-González, S Molin

Abstract

A gene homologous to the rpoS gene of Escherichia coli was cloned from a Pseudomonas putida KT2440 gene bank by complementation of the rpoS-deficient strain E. coli ZK918. The rpoS gene of P. putida complemented the acid sensitivity and catalase deficiency of the rpoS mutant of E. coli and stimulated expression of the RpoS-controlled promoter, bolAp1. The gene was sequenced and found to be highly similar to the rpoS genes of other gram-negative bacteria. Like in other gram-negative bacteria, a homolog of the nlpD gene was found upstream to the rpoS gene. A transcriptional fusion of the promoter of the P. putida rpoS gene to the luxAB genes from Vibrio harveyi was constructed and used as an inactivated allele of rpoS for gene replacement of the wild-type copy in the chromosome of P. putida. The resultant rpoS mutant of P.putida, C1R1, showed reduced survival of carbon starvation and reduced cross-protection against other types of stress in cells starved for carbon, in particular after a challenge with ethanol. Survival in soil amended with m-methylbenzoate was also reduced in the mutant strain P. putida C1R1. The RpoS protein of P. putida controls the expression of more than 50 peptides, which are normally expressed in cells aft...Continue Reading

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Citations

Dec 26, 2001·FEMS Microbiology Letters·H AramakiY Sagara
Oct 9, 2003·Applied and Environmental Microbiology·A HülsmannJ D Oliver
Sep 25, 2003·Antimicrobial Agents and Chemotherapy·Wilson TeránMaría-Trinidad Gallegos
Aug 22, 2009·FEMS Microbiology Letters·Andreas PetersenJohn Elmerdahl Olsen
Oct 30, 2010·Microbiology·Geneviève Girard, Sébastien Rigali
Jun 1, 2011·FEMS Microbiology Letters·Cecilia PiniAna Segura
Apr 16, 2013·Environmental Microbiology Reports·Emma SevillaFernando Rojo
Nov 20, 2013·Environmental Microbiology Reports·Juhyun KimRafael Silva-Rocha
Sep 17, 2015·Environmental Microbiology·Alejandro Arce-RodríguezVíctor de Lorenzo
Sep 6, 2003·Applied and Environmental Microbiology·María-Isabel Ramos-GonzálezJuan L Ramos
Sep 4, 2004·Applied and Environmental Microbiology·Manuel Espinosa-Urgel, Juan-Luis Ramos
Mar 31, 2004·Canadian Journal of Microbiology·C D MillerA J Anderson
Dec 1, 2000·Applied and Environmental Microbiology·S VílchezJ L Ramos
Jul 1, 2005·Critical Reviews in Microbiology·Daniel KadouriSusana Castro-Sowinski
Feb 26, 2016·Environmental Microbiology·Eugeni BeldaClaudine Médigue
Oct 16, 2015·Environmental Microbiology·David Martín-MoraTino Krell
Jul 27, 2001·Applied and Environmental Microbiology·B KochO Nybroe

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