Cloning, sequencing and proposed structure for a prostaglandin F2 alpha receptor regulatory protein

Prostaglandins, Leukotrienes, and Essential Fatty Acids
D J Orlicky, S K Nordeen

Abstract

The cDNA has been cloned for a protein which copurifies with and colocalizes with [3H]PGF2 alpha binding activity, yet differs from the previously cloned prostaglandin F2 alpha receptor. Polyclonal antibody, produced against one of two protein bands present in a highly purified preparation of [3H]PGF2 alpha binding activity isolated from pregnant bovine corpus luteum, was used to screen a rat ovary cDNA expression library. A single strongly positive clone was identified containing a 4 kilobase (kb) insert. Northern analysis using this cDNA as a probe revealed the expression of a 6 kb mRNA with a tissue distribution similar to that seen by immunohistochemical analysis with the polyclonal antibody. Tissues possessing the largest quantity of the protein's mRNA are reproductive tissues, lung, and heart. Directed cDNA synthesis was required to clone the 5' end of the cDNA. Verification that the correct cDNA was cloned is provided by alignment of the predicted protein's mature amino terminal amino acid sequence with sequence observed by protein sequencing. Translation of the predicted 879 amino acid open reading frame (ORF) suggests a protein structure exhibiting six glycosylated immunoglobulin type loops, one of which may either be ...Continue Reading

References

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Dec 1, 1992·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D J Orlicky, C Williams-Skipp
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Citations

May 20, 2003·The British Journal of Dermatology·M BonkobaraK Ariizumi
Apr 1, 1996·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D J Orlicky
Nov 24, 1999·Biochimica Et Biophysica Acta·A V TkatchenkoC A Dechesne
Mar 30, 2001·The Journal of Biological Chemistry·S CharrinE Rubinstein
Apr 12, 2013·Nature Communications·Stéphanie CharrinEric Rubinstein
Jul 14, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L WengJ Merregaert
Nov 23, 2000·The Journal of Biological Chemistry·C S StippM E Hemler
Feb 24, 2005·The Biochemical Journal·Christoph ClaasMargot Zöller
Jul 20, 2006·Cancer Research·Sabine GesierichMargot Zöller

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