Cloning the genes and DNA binding properties of High Mobility Group B1 (HMGB1) proteins from the human blood flukes Schistosoma mansoni and Schistosoma japonicum

Gene
Francisco Meirelles Bastos de OliveiraMarcelo Rosado Fantappié

Abstract

The parasitic helminth Schistosoma mansoni contains three HMGB proteins, HMGB1, HMGB2 and HMGB3, of primary amino acid sequences highly similar to vertebrate proteins. In this report we describe the characterization of the HMGB1 proteins and their genes from S. mansoni and Schistosoma japonicum. The deduced amino acid sequences of HMGB1 proteins from both schistosome species are identical, and comprise 176 residues. The proteins contain the two evolutionarily highly conserved HMG-box domains, A and B, exhibiting 60% similarity to mammalian HMGB1. Unlike the human HMGB1 which contains an unbroken run of 30 glutamic or aspartic residues, the SmHMGB1 or SjHMGB1 proteins possess unusually short acidic C-terminal tails (5 acidic residues interrupted by 2 serines). Southern hybridization and DNA sequencing revealed a single copy HMGB1 gene, composed of 3 exons and two introns, in S. mansoni. The exon/intron boundaries are identical to those of the human HMGB1 gene, with the exception that the second exon of the SmHMGB1 gene which is not split into two exons as in the human HMGB1 gene. RNA blot analysis revealed that the SmHMGB1 gene is constitutively expressed in similar levels both in male and female worms. The single-sized mRNA for...Continue Reading

References

Jun 1, 1992·Journal of Cellular Biochemistry·E M RabeloF D Rumjanek
Feb 24, 1989·Science·M E BianchiG Paonessa
May 11, 1995·Nucleic Acids Research·A D Baxevanis, D Landsman
Jan 1, 1994·Parasitology·M R Fantappié, F D Rumjanek
Jan 18, 1994·Biochemistry·L A Kohlstaedt, R D Cole
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P M PilS J Lippard
Mar 14, 1998·The EMBO Journal·J R P-ohlerD M Lilley
Jul 8, 1998·European Journal of Biochemistry·K D GrasserJ O Thomas
Jul 10, 1999·Science·H WangK J Tracey
Dec 3, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M R FantappiéF D Rumjanek
Feb 19, 2000·Current Opinion in Structural Biology·A Travers
Jun 24, 2000·European Journal of Biochemistry·M StrosT M Jovin
Mar 14, 2001·Trends in Biochemical Sciences·J O Thomas, A A Travers
Dec 18, 2002·The EMBO Journal·Tiziana BonaldiMarco E Bianchi
Mar 4, 2003·EMBO Reports·Andrew A Travers
Apr 4, 2003·Current Opinion in Genetics & Development·Alessandra Agresti, Marco E Bianchi
Sep 16, 2003·Nature Genetics·Sergio Verjovski-AlmeidaEmmanuel Dias-Neto
Mar 24, 2004·International Journal for Parasitology·Donald P McManusDavid Blair
Apr 15, 2004·Parasitology International·Donald P McManus, Paul B Bartley
Apr 22, 2004·Trends in Parasitology·Najib M A El-SayedPhilip T LoVerde
Jun 15, 2004·Trends in Parasitology·Sergio Verjovski-AlmeidaR Alan Wilson
Apr 2, 2005·Nature Reviews. Immunology·Michael T Lotze, Kevin J Tracey
Jun 1, 2005·Trends in Parasitology·Julius LukesTomás Scholz
Jun 9, 2005·Biochemical and Biophysical Research Communications·Ricardo DeMarcoSergio Verjovski-Almeida
Oct 26, 2005·Molecular and Biochemical Parasitology·Munirathinam GnanasekarKalyanasundaram Ramaswamy

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Citations

Jan 29, 2016·International Journal for Parasitology·Isabel Caetano de Abreu da SilvaMarcelo Rosado Fantappié
Jan 15, 2008·Acta Tropica·Marcelo Rosado FantappíéFranklin David Rumjanek
Nov 3, 2009·Biochemical and Biophysical Research Communications·Vitor Coutinho CarneiroMarcelo Rosado Fantappié
Jul 11, 2014·Molecular Aspects of Medicine·Rui KangDaolin Tang
Mar 15, 2012·Molecular and Biochemical Parasitology·Letícia AndersonSergio Verjovski-Almeida
Dec 6, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Tianzhu ZhangJuan Du
Feb 9, 2017·PLoS Neglected Tropical Diseases·Pamela CribbRogelio Hernández-Pando

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