Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae

Gene
P K Foreman, R W Davis

Abstract

A series of cloning vectors, designated YCpIF, was constructed to facilitate the conditional synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. These vectors contain a translation start codon upstream from a multiple cloning site (MCS) in each of the three reading frames. Protein synthesis is under the control of the GAL1 promoter, which drives transcription when cells are grown on galactose-containing medium, but not when they are grown on glucose-containing medium. Different versions of the vectors contain four different commonly used selectable markers. In addition, YCpIF15, YCpIF16 and YCpIF17 contain a sequence encoding an epitope from influenza virus hemagglutinin upstream from the MCS. These vectors facilitate the addition of this epitope tag to the N terminus of any protein. The epitope is recognized by a commercially available monoclonal antibody.

References

Aug 1, 1992·Journal of Virology·J LubanS P Goff
Jan 1, 1991·Methods in Enzymology·P A Kolodziej, R A Young
Jan 1, 1991·Methods in Enzymology·F Sherman
Aug 1, 1984·Molecular and Cellular Biology·M Johnston, R W Davis
Jul 1, 1984·Cell·I A WilsonR A Lerner
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S W Ramer, R W Davis

❮ Previous
Next ❯

Citations

May 23, 1996·Molecular & General Genetics : MGG·W Jiang, Y Koltin
Jan 29, 2000·Molecular Microbiology·A SonnebornJ F Ernst
Jul 24, 2013·The Journal of Cell Biology·Masayuki OnishiJohn R Pringle
Jun 26, 2004·Molecular Biology of the Cell·Thomas DoedtJoachim F Ernst
Aug 22, 2001·Molecular Biology of the Cell·H A HarkinsJ R Pringle
May 16, 2008·Molecular Biology of the Cell·Jennifer R LarsonKelly Tatchell
May 11, 2000·Molecular and Cellular Biology·E Bilsland-MarchesanF Posas
Feb 3, 1999·Annual Review of Genetics·J W Jarvik, C A Telmer
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·D YablonskiA Levitzki
May 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Diego WengierJorge Muschietti
Dec 18, 2013·Biotechnology Advances·Ana Sofia PinaAna Cecília A Roque
Aug 23, 1996·Cell·J Y ThuretC Mann
Feb 28, 1998·The Journal of Cell Biology·R K Miller, M D Rose
Feb 4, 2009·The Journal of Cell Biology·Casey A Ydenberg, Mark D Rose
Oct 22, 2016·PLoS Genetics·Lasse van WijlickJoachim F Ernst
Jun 19, 2003·The Journal of Biological Chemistry·Carlos J Rodriguez-HernandezJosé R Murguia
Mar 30, 2001·The Journal of Biological Chemistry·K BaudryJ T Nickels
Nov 7, 2019·ELife·Andrea BrennaUrs Albrecht
Feb 12, 2008·Current Protocols in Molecular Biology·P B Ouwerkerk, A H Meijer
Aug 29, 1997·Science·D A SinclairL Guarente

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.