Clonogenic assay allows for selection of a primitive mammary epithelial cell population in bovine

Experimental Cell Research
Eugenio MartignaniMario Baratta

Abstract

Adult mammary stem cells have been identified in several species including the bovine. They are responsible for the development of the gland and for cyclic remodeling during estrous cycles and pregnancy. Epithelial cell subpopulations exist within the mammary gland. We and others showed previously that the Colony Forming Cell (CFC) assay can be used to detect lineage-restricted mammary progenitors. We carried out CFCs with bovine mammary cells and manually separated colonies with specific morphologies associated with either a luminal or a myoepithelial phenotype. Expression of specific markers was assessed by immunocytochemistry or by flow cytometry to confirm that the manual separation resulted in isolation of phenotipically different cells. When transplanted in recipient immunodeficient mice, we found that only myoepithelial-like colonies gave rise to outgrowths that resembled bovine mammary alveoli, thus proving that adult stem cells were maintained during culture and segregated with myoepithelial cells. After recovery of the cells from the transplanted mice and subsequent progenitor content analysis, we found a tendency to detect a higher progenitor frequency when myoepithelial-like colonies were transplanted. We here demon...Continue Reading

References

May 12, 2005·Journal of Mammary Gland Biology and Neoplasia·John StinglConnie J Eaves
May 12, 2005·Journal of Mammary Gland Biology and Neoplasia·Agla Jael Rubner FridriksdottirOle William Petersen
Jan 6, 2006·Nature·John StinglConnie J Eaves
Jan 7, 2006·Nature·Mark ShackletonJane E Visvader
Nov 15, 2008·The Journal of Pathology·J Stingl
Jul 7, 2009·Veterinary Research Communications·E MartignaniM Baratta
Nov 10, 2009·Breast Cancer Research and Treatment·L HankerA Rody
Jan 13, 2012·Biology of Reproduction·Sonja PrparMario Baratta
Jan 19, 2012·PloS One·Gat Rauner, Itamar Barash
Jan 28, 2014·Nature·Anne C RiosJane E Visvader
Sep 1, 2014·Nature Cell Biology·Michael D PraterJohn Stingl
Mar 12, 2015·Journal of Biomolecular Structure & Dynamics·Neelesh SharmaDong Kee Jeong

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Citations

Jan 27, 2017·American Journal of Respiratory Cell and Molecular Biology·Preston J AndersonJohn F Engelhardt
Nov 21, 2017·The Journal of Dairy Research·Mario BarattaEugenio Martignani

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