Clonotypic analysis of anti-acetylcholine receptor antibodies produced against native and denatured antigen.

Journal of Neuroimmunology
T M Yeh, K A Krolick

Abstract

Rats were immunized with either conformationally intact acetylcholine receptor (AChR) or reduced and sodium dodecyl sulfate (SDS)-denatured AChR. As expected, challenge with native AChR (nAChR) resulted in the production of serum antibodies reactive with native AChR; these antibodies were, as observed in earlier studies, oligoclonal, dominated by the rat IgG2a subclass, heterogeneous with respect to binding avidity, and importantly, able to interfere with normal AChR-dependent muscle contraction. Antibodies produced as a result of immunization with denatured AChR (dAChR) that were crossreactive with nAChR were similar but not identical to those produced directly against nAChR; in contrast, however, dAChR-stimulated antibodies were clearly incapable of causing detectable impairment of AChR-dependent muscle contractile function. This difference in disease-causing potential was demonstrated in spite of the observation that either nAChR or dAChR could generate similar levels of circulating antibodies that reacted with the native form of AChR. Moreover, the same difference in disease-causing potential was again observed upon passive intravenous administration of anti-AChR antibodies into naive recipient rats. IEF analyses demonstrat...Continue Reading

References

Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D Bartfeld, S Fuchs
Jan 1, 1976·Annals of the New York Academy of Sciences·R ValderramaA Karlin
Jan 1, 1976·Annals of the New York Academy of Sciences·J M LindstromS Whittingham
Jan 1, 1981·Annual Review of Neuroscience·D B Drachman

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Citations

Jan 1, 1991·Preparative Biochemistry·P A Thompson, K A Krolick
Jun 21, 1993·Annals of the New York Academy of Sciences·K A KrolickT M Yeh
Apr 28, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Sabrina ShandleyKeith Krolick
Jan 19, 2000·Clinical Immunology : the Official Journal of the Clinical Immunology Society·T Stegall, K A Krolick
Jan 1, 1992·Drug and Chemical Toxicology·T M YehK A Krolick
Jan 1, 1992·Muscle & Nerve·P A ThompsonK A Krolick

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