Clonotypic chromosomal aberrations in long-term lines of myelin-specific rat T lymphocytes.

Journal of Neuroimmunology
M BradlH Wekerle

Abstract

A panel of 16 long-term rat T lymphocyte lines and clones were screened for cytogenetical abnormalities using chromosomal banding techniques. All T lines were CD4+, recognizing the relevant antigen in the molecular context of major histocompatibility complex (MHC) class II determinants. With one exception (an ovalbumin-specific line), all lines were specific for myelin proteins, and apart of one BS rat-derived T line and its clones, all lines were selected from the Lewis strain of rat. After in vitro culture of more than 1 year, all lines and clones exhibited subtle but definite chromosomal aberrations, which included deletions, enlargement, translocations and formation of isochromosomes. All lines were near diploid, structural chromosomal changes being more frequent than numerical abnormalities. Each T line investigated had an individual pattern of chromosomal changes. In our analysis, 16 of the 22 different chromosomes had changes in at least one line. Chromosome 9 and the X chromosome appeared to have an enhanced susceptibility of alterations. In two cases, chromosomal markers could be traced through different stages of in vitro culture of the T lines.

References

Jul 30, 1977·Lancet
Mar 1, 1979·Mechanisms of Ageing and Development·L Hayflick
Mar 15, 1979·International Journal of Cancer. Journal International Du Cancer·G Kovacs
Feb 1, 1989·European Journal of Immunology·J ChlubaJ T Epplen
Jul 1, 1985·Cancer Genetics and Cytogenetics·N SadamoriM Ichimaru
Oct 1, 1986·European Journal of Immunology·M M SimonJ T Epplen
Jan 1, 1987·Annual Review of Immunology·B Toyonaga, T W Mak
Nov 1, 1987·Experimental Eye Research·J T Den DunnenJ G Schoenmakers
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R TakahashiT Sugiyama
Jan 1, 1985·Somatic Cell and Molecular Genetics·J SzpirerC Szpirer
Oct 30, 1971·Lancet·M Seabright
Nov 1, 1972·Experimental Cell Research·A T Sumner
Feb 1, 1982·Cancer Genetics and Cytogenetics·P E Barker
Jan 1, 1984·Human Immunology·R B Effros, R L Walford
Mar 14, 1956·Annals of the New York Academy of Sciences·A LEVAN
Mar 1, 1965·Experimental Cell Research·L HAYFLICK

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.