Clonotypic Diversity of the T-cell Receptor Corroborates the Immature Precursor Origin of Cutaneous T-cell Lymphoma

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Abdelbasset HamrouniRobert Gniadecki

Abstract

Mycosis fungoides is one of the most common types of extranodal T-cell lymphomas, considered to be caused by malignant transformation of the mature T cells residing in the skin. However, some clinical observations such as the multifocal distribution of mycosis fungoides lesions or patterns of relapse after radiotherapy are not readily explainable by the mature T-cell origin theory. We have performed a detailed analysis of T-cell receptor (TCR) rearrangements in single malignant cells and in biopsies from mycosis fungoides tumors composed of >80% of malignant cells using next-generation sequencing (NGS) to pinpoint the relationship between neoplastic cells in mycosis fungoides. We have also aimed to detect malignant, circulating T-cell by whole blood TCR sequencing. We found a substantial clonal heterogeneity in the mycosis fungoides samples with regards to TCR, and we demonstrated that lymphoma cells harboring identical TCRγ sequences may harbor different TCRα and β sequences. Lack of absolute TCRα, -β, -γ monoclonality was further confirmed by TCR amplification and sequencing from microdissected lymphoma cells. We have also found the TCR rearrangements characteristic for lymphoma cells in patients' peripheral blood despite the...Continue Reading

References

Mar 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K KaltoftK Thestrup-Pedersen
Aug 4, 1988·Nature·M M Davis, P J Bjorkman
Apr 14, 1983·Nature·S Tonegawa
Jan 17, 2002·The New England Journal of Medicine·K D BergJ R Eshleman
Jun 25, 2002·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·Günter BurgReinhard Dummer
Mar 5, 2003·The Journal of Experimental Medicine·Abdelbasset HamrouniJanet L Maryanski
Jul 5, 2003·Blood·Robert GniadeckiHans Christian Wulf
Feb 5, 2005·Blood·Rein WillemzeChris J L M Meijer
Jun 21, 2005·Journal of the American Academy of Dermatology·Julie C SchultzSam T Hwang
Sep 23, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·David H Wagner
Mar 16, 2007·Experimental Dermatology·Maria R KamstrupGunhild L Skovgaard
Jun 24, 2014·Nature Biotechnology·Arnold HanMark M Davis
Aug 21, 2014·The American Journal of Dermatopathology·Panagiota MantakaJan Delabie
Dec 31, 2014·The American Journal of Dermatopathology·Mariela Judith NevetReuven Bergman
Jan 27, 2015·Cellular & Molecular Immunology·Meriem AttafAndrew K Sewell
Apr 30, 2015·Nature Methods·Dmitriy A BolotinDmitriy M Chudakov
Jun 7, 2015·Journal of the American Academy of Dermatology·Kari E SufficoolEric J Duncavage
Jul 8, 2015·Nature Medicine·Catherine B Meador, Christine M Lovly
Jul 21, 2015·Nature Genetics·Jaehyuk ChoiRichard P Lifton
Sep 2, 2015·Nature Communications·Eliana RuggieroChristof von Kalle
Oct 9, 2015·Science Translational Medicine·Ilan R KirschRachael A Clark
Nov 26, 2015·PLoS Computational Biology·Mikhail ShugayDmitriy M Chudakov
Apr 16, 2016·Blood·Mark RoschewskiWyndham H Wilson
Jun 28, 2016·Journal of the European Academy of Dermatology and Venereology : JEADV·L Y McGirtC M Eischen
May 24, 2017·The Journal of Investigative Dermatology·Tiago R MatosMarcel B M Teunissen
Apr 29, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christopher J Gibson, David P Steensma
May 11, 2018·Science Translational Medicine·Adele de MassonThomas S Kupper

❮ Previous
Next ❯

Citations

Apr 25, 2019·Toxicological Research·Jung Dae LeeKyu-Bong Kim
Jul 30, 2019·Journal of Cutaneous Medicine and Surgery·Arunima SivanandRobert Gniadecki
May 16, 2020·Blood Cancer Journal·Andreas Willerslev-OlsenNiels Ødum
Dec 18, 2020·Frontiers in Immunology·Arunima SivanandRobert Gniadecki
Jun 10, 2020·Cold Spring Harbor Perspectives in Medicine·José R Cortés, Teresa Palomero

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Sequencing

Several sequencing approaches are employed to understand and examine tumor development and progression. These include whole genome as well as RNA sequencing. Here is the latest research on cancer sequencing.

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.

© 2022 Meta ULC. All rights reserved