Clopidogrel reduces apoptosis and promotes proliferation of human vascular endothelial cells induced by palmitic acid via suppression of the long non-coding RNA HIF1A-AS1 in vitro

Molecular and Cellular Biochemistry
Jing WangXueling Zhao

Abstract

Cardiovascular disease (CVD) is recognized as a major and increasing health problem affected older subjects in China, and clopidogrel has been widely used for treatment of CVD patients such as atherosclerosis, myocardial infarction, and myocardial ischaemia-reperfusion damage. However, the molecular mechanisms of clopidogrel for treatment of CVD are only partially understood. This study investigated the effects of clopidogrel on palmitic acid-induced damage of human vascular endothelial cells (HUVECs), and the molecular mechanisms of LncRNA HIF1A-AS1 in regulating the proliferation and apoptosis of HUVECs in vitro. We firstly established a damage model of HUVECs through palmitic acid (PA) treatment. And the effect of clopidogrel reducing PA-induced apoptosis of HUVECs was observed by the flow cytometric measurement. To further understand the molecular mechanism of clopidogrel rescues PA-induced apoptosis, we used human LncRNA PCR array to compare the LncRNA expression profile difference between clopidogrel-treated cells and control cells. The expression of LncRNA HIF 1 alpha-antisense RNA 1 (HIF1A-AS1) was significantly altered in clopidogrel-treated cells. We further proved that suppression of HIF1A-AS1 by siRNA reduce PA-indu...Continue Reading

References

Jan 14, 1999·The New England Journal of Medicine·R Ross
Sep 23, 2000·Nature·A J Lusis
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nabil G SeidahMichel Chretien
Jun 30, 2004·Cell Biology International·Ridvan YalcinErkan Yurtcu
Dec 17, 2005·Antioxidants & Redox Signaling·Cindy J M LoomansAnton-Jan Van Zonneveld
May 3, 2006·Human Molecular Genetics·John S Mattick, Igor V Makunin
Nov 8, 2006·Circulation·Yangfeng WuUNKNOWN China Multicenter Collaborative Study of Cardiovascular Epidemiology Research Group
May 23, 2009·Journal of the American College of Cardiology·Colin M Barker, H Vernon Anderson
Oct 23, 2010·Pharmacology & Therapeutics·Hong-Guang XieShao-Liang Chen
Apr 30, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Sohsuke YamadaYasuyuki Sasaguri
Feb 14, 2013·Primary Care·Robert H Nelson
Feb 16, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Qunhua HuangWang Min
Apr 24, 2014·Materia Socio-medica·Merita Tiric CamparaAzra Alajbegovic
May 31, 2014·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Shuwei WangZhiyun Xu

❮ Previous
Next ❯

Citations

Mar 21, 2017·The EMBO Journal·Roberto VendraminEleonora Leucci
May 30, 2015·Cellular and Molecular Life Sciences : CMLS·Joseph M Miano, Xiaochun Long
Dec 25, 2015·Journal of Atherosclerosis and Thrombosis·Liguo JianLi Zhang
Sep 28, 2017·Cancer Biomarkers : Section a of Disease Markers·Wanjun GongZhenhua Yang
Dec 12, 2018·Cell & Bioscience·Hu PengJiang-Feng Wu
Jan 31, 2020·Oxidative Medicine and Cellular Longevity·Qidong CaoChunli Song
Feb 3, 2019·Non-coding RNA·Shrey GandhiMonika Stoll
Nov 17, 2020·Frontiers in Physiology·Qingyu MengBin Liu
Nov 26, 2020·Experimental and Molecular Pathology·Soudeh Ghafouri-FardMohammad Taheri
Jul 11, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hao HuLikun Ma

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis