Abstract
Determining histologic types of lung cancer on biopsies can be difficult. This study addresses the role of immunohistochemistry in histologic typing, using a tissue microarray (TMA) as "model biopsies," and presents a classification generated by an unsupervised hierarchical cluster analysis. A TMA was made from resection specimens of a consecutive series of 165 lung tumors. In a "tissue-spot review" with hematoxylin and eosin sections all the large cell carcinomas (N=22) were assigned to the noncommittal class of non-small cell lung cancer (NSCLC), as were an additional 37 tumors of defined histologic types. Adenocarcinomas and squamous cell carcinomas included with these NSCLC could be diagnosed by immunohistochemistry with antibodies against TTF-1, Napsin A, cytokeratin (CK)7, p40, p63, and CK5/6 with moderate to good sensitivities and specificities. Unsupervised hierarchical clustering was done with these data and additional high-molecular-weight cytokeratins, CD56, synaptophysin, and chromogranin immunohistochemistry. This delineated separate clusters for adenocarcinomas, large cell carcinomas, neuroendocrine tumors, and squamous cell carcinomas. Notably, adenocarcinoma and large cell carcinoma clusters were closely related...Continue Reading
References
Nov 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·A BhattacharjeeM Meyerson
Feb 27, 2003·The American Journal of Surgical Pathology·Robert W WerlingAllen M Gown
Aug 13, 2004·The Journal of Pathology·N H C AuH L Grimes
May 12, 2009·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Brian Z RingDouglas T Ross
Feb 23, 2010·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Michael Yang, Daisuke Nonaka
Nov 26, 2010·The American Journal of Surgical Pathology·Jefferson TerryDiana N Ionescu
Dec 18, 2010·The American Journal of Surgical Pathology·Sanjay Mukhopadhyay, Anna-Luise A Katzenstein
Jan 22, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·William D TravisDavid Yankelewitz
Apr 23, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Giuseppe PelosiGiuseppe Viale
Aug 14, 2012·Histopathology·Arne WarthPhilipp A Schnabel
Dec 1, 2012·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Natasha RekhtmanMarc Ladanyi
Mar 15, 2013·International Journal of Surgical Pathology·Giuseppe PelosiMauro Papotti
Sep 28, 2013·Nature Genetics·Giovanni CirielloChris Sander
Nov 14, 2013·Virchows Archiv : an International Journal of Pathology·G RossiG Pelosi
Citations
Mar 30, 2019·Journal for Immunotherapy of Cancer·Anne MonetteRéjean Lapointe
Sep 27, 2019·Acta Biochimica Et Biophysica Sinica·Yifeng GongYan Jin
Jan 23, 2016·Hematological Oncology·Thomas MenterAlexandar Tzankov