Closed-form density-based framework for automatic detection of cellular morphology changes.

Proceedings of the National Academy of Sciences of the United States of America
Tarn DuongKristine Schauer

Abstract

A primary method for studying cellular function is to examine cell morphology after a given manipulation. Fluorescent markers attached to proteins/intracellular structures of interest in conjunction with 3D fluorescent microscopy are frequently exploited for functional analysis. Despite the central role of morphology comparisons in cell biological approaches, few statistical tools are available that allow biological scientists without a high level of statistical training to quantify the similarity or difference of fluorescent images containing multifactorial information. We transform intracellular structures into kernels and develop a multivariate two-sample test that is nonparametric and asymptotically normal to directly and quantitatively compare cellular morphologies. The asymptotic normality bypasses the computationally intensive calculations used by the usual resampling techniques to compute the P-value. Because all parameters required for the statistical test are estimated directly from the data, it does not require any subjective decisions. Thus, we provide a black-box method for unbiased, automated comparison of cell morphology. We validate the performance of our test statistic for finite synthetic samples and experimen...Continue Reading

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Citations

Feb 8, 2014·PloS One·Jasmine Burguet, Philippe Andrey
Dec 10, 2014·The Journal of Cell Biology·Marcel ReuterClaire Wyman
Apr 27, 2016·BMC Bioinformatics·Yingbo LiAuguste Genovesio
Apr 29, 2016·The Plant Journal : for Cell and Molecular Biology·Eric BiotPhilippe Andrey
Nov 20, 2016·Molecular Ecology·Wesley A LarsonLisa W Seeb
Jan 15, 2016·Journal of Cell Science·Maya SpichalEmmanuelle Fabre
Nov 16, 2018·Current Protocols in Cell Biology·Anahi CapmanyKristine Schauer
Mar 16, 2019·Journal of Cell Science·Anahi CapmanyKristine Schauer
Nov 8, 2019·Biometrical Journal. Biometrische Zeitschrift·Polychronis EconomouUNKNOWN Alzheimer's Disease Neuroimaging Initiative
Aug 21, 2013·Journal of Biomolecular Screening·Kristine SchauerBruno Goud
Jan 7, 2021·Journal of the Royal Society, Interface·Alexander B BrummerVan M Savage
Jan 16, 2021·Journal of Evolutionary Biology·Tsuyoshi ItoSuchinda Malaivijitnond
Nov 27, 2021·Nature Communications·Yue CaoJean Yee Hwa Yang
Jul 25, 2019··Tyler ThrashJascha Grübel

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