Clostridium difficile: Investigating Transmission Patterns Between Infected and Colonized Patients Using Whole Genome Sequencing.

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
Ling Yuan KongVivian G Loo

Abstract

Whole genome sequencing (WGS) studies can enhance our understanding of the role of patients with asymptomatic Clostridium difficile colonization in transmission. Isolates obtained from patients with Clostridium difficile infection (CDI) and colonization identified in a study conducted during 2006-2007 at 6 Canadian hospitals underwent typing by pulsed-field gel electrophoresis, multilocus sequence typing, and WGS. Isolates from incident CDI cases not in the initial study were also sequenced where possible. Ward movement and typing data were combined to identify plausible donors for each CDI case, as defined by shared time and space within predefined limits. Proportions of plausible donors for CDI cases that were colonized, infected, or both were examined. Five hundred fifty-four isolates were sequenced successfully, 353 from colonized patients and 201 from CDI cases. The NAP1/027/ST1 strain was the most common strain, found in 124 (62%) of infected and 92 (26%) of colonized patients. A donor with a plausible ward link was found for 81 CDI cases (40%) using WGS with a threshold of ≤2 single nucleotide polymorphisms to determine relatedness. Sixty-five (32%) CDI cases could be linked to both infected and colonized donors. Exclusi...Continue Reading

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Citations

Jan 11, 2019·Microbial Genomics·Yasuhiro GotohTetsuya Hayashi
Jan 25, 2020·Expert Review of Anti-infective Therapy·Adriana M RauseoErik R Dubberke
Jul 18, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jay WorleyLynn Bry
May 31, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Justin J O'Hagan, L Clifford McDonald
Jun 6, 2020·Nature Reviews. Microbiology·Thea Van RossumPeer Bork
Mar 9, 2021·Expert Review of Molecular Diagnostics·Masako Mizusawa, Karen C Carroll
Aug 8, 2021·Infectious Disease Clinics of North America·Philip C Carling

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