Clostridium perfringens Virulent Bacteriophage CPS2 and Its Thermostable Endolysin LysCPS2

Viruses
Eunsu HaSangryeol Ryu

Abstract

Clostridium perfringens is one of the most common causes of food-borne illness. The increasing prevalence of multidrug-resistant bacteria requires the development of alternatives to typical antimicrobial treatments. Here, we isolated and characterized a C. perfringens-specific virulent bacteriophage CPS2 from chicken feces. The CPS2 phage contains a 17,961 bp double-stranded DNA genome with 25 putative ORFs, and belongs to the Picovirinae, subfamily of Podoviridae. Bioinformatic analysis of the CPS2 genome revealed a putative endolysin, LysCPS2, which is homologous to the endolysin of Clostridium phage phiZP2 and phiCP7R. The enzyme showed strong lytic activity against C. perfringens with optimum conditions at pH 7.5⁻10, 25⁻65 °C, and over a broad range of NaCl concentrations. Interestingly, LysCPS2 was found to be highly thermostable, with up to 30% of its lytic activity remaining after 10 min of incubation at 95 °C. The cell wall binding domain in the C-terminal region of LysCPS2 showed a binding spectrum specific to C. perfringens strains. This is the first report to characterize highly thermostable endolysin isolated from virulent C. perfringens bacteriophage. The enzyme can be used as an alternative biocontrol and detectio...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 20, 2003·Journal of Bacteriology·Daniel NelsonVincent A Fischetti
Jul 17, 2004·Microbiology·David G PritchardJeffrey A Engler
Nov 19, 2004·Cellular and Molecular Life Sciences : CMLS·R LavigneG Volckaert
Jun 28, 2005·Current Opinion in Microbiology·Martin J Loessner
Mar 28, 2006·Experimental Biology and Medicine·Jan BorysowskiAndrzej Górski
Feb 12, 2008·BMC Genomics·Ramy K AzizOlga Zagnitko
Apr 13, 2011·Applied Microbiology and Biotechnology·Hirofumi NariyaAkinobu Okabe
Aug 5, 2011·Avian Pathology : Journal of the W.V.P.A·L TimbermontF Van Immerseel
Jul 27, 2012·Applied Microbiology and Biotechnology·Maarten WalmaghRob Lavigne
Oct 4, 2012·Future Microbiology·Mathias SchmelcherMartin J Loessner
Aug 15, 2013·Applied Microbiology and Biotechnology·Teresa GervasiMelinda J Mayer
Jun 6, 2015·European Biophysics Journal : EBJ·Minsuk KongSangryeol Ryu

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Citations

Aug 14, 2019·Viruses·Sílvio B Santos, Joana Azeredo
Nov 14, 2019·Antibiotics·Steven M SwiftTimothy G Ramsay
Dec 11, 2020·Scientific Reports·Zhen ZhangMiia Lindström
Nov 13, 2019·International Journal of Biological Macromolecules·Ramachandran SrinivasanKodiveri Muthukaliannan Gothandam

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Datasets Mentioned

BETA
MH248069

Methods Mentioned

BETA
Whole Genome Sequencing
PCR
Fluorescence
electrophoresis

Software Mentioned

Macrogen
BLAST
Rapid Annotation using Subsystem Technology ( RAST )
InterProScan
GS De Novo Assembler
BLASTP
Glimmer

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