Closure of VDAC causes oxidative stress and accelerates the Ca(2+)-induced mitochondrial permeability transition in rat liver mitochondria.

Archives of Biochemistry and Biophysics
Andrey TikunovEkhson Holmuhamedov

Abstract

The electron transport chain of mitochondria is a major source of reactive oxygen species (ROS), which play a critical role in augmenting the Ca(2+)-induced mitochondrial permeability transition (MPT). Mitochondrial release of superoxide anions (O(2)(-)) from the intermembrane space (IMS) to the cytosol is mediated by voltage dependent anion channels (VDAC) in the outer membrane. Here, we examined whether closure of VDAC increases intramitochondrial oxidative stress by blocking efflux of O(2)(-) from the IMS and sensitizing to the Ca(2+)-induced MPT. Treatment of isolated rat liver mitochondria with 5microM G3139, an 18-mer phosphorothioate blocker of VDAC, accelerated onset of the MPT by 6.8+/-1.4min within a range of 100-250microM Ca(2+). G3139-mediated acceleration of the MPT was reversed by 20microM butylated hydroxytoluene, a water soluble antioxidant. Pre-treatment of mitochondria with G3139 also increased accumulation of O(2)(-) in mitochondria, as monitored by dihydroethidium fluorescence, and permeabilization of the mitochondrial outer membrane with digitonin reversed the effect of G3139 on O(2)(-) accumulation. Mathematical modeling of generation and turnover of O(2)(-) within the IMS indicated that closure of VDAC pr...Continue Reading

References

Dec 11, 1975·Biochimica Et Biophysica Acta·J ButlerA J Swallow
Jul 1, 1979·Archives of Biochemistry and Biophysics·R A Haworth, D R Hunter
Jul 1, 1979·Archives of Biochemistry and Biophysics·D R Hunter, R A Haworth
Feb 1, 1991·International Journal of Radiation Biology·B H Bielski, D E Cabelli
May 1, 1990·The American Journal of Physiology·T E Gunter, D R Pfeiffer
Oct 1, 1986·Journal of Bioenergetics and Biomembranes·C R HackenbrockS S Gupte
Jul 1, 1968·The Journal of Cell Biology·C Schnaitman, J W Greenawalt
Apr 1, 1993·The American Journal of Physiology·T L DawsonJ J Lemasters
Apr 1, 1997·The American Journal of Physiology·A L NieminenJ J Lemasters
Feb 1, 1997·Bioscience Reports·J F Turrens
Oct 18, 2000·Free Radical Biology & Medicine·E Cadenas, K J Davies
Aug 17, 2001·The Journal of Biological Chemistry·A Okado-Matsumoto, I Fridovich
May 9, 2002·Journal of the American Chemical Society·Gianantonio BattistuzziMarco Sola
Dec 17, 2002·The Journal of Biological Chemistry·Derick HanEnrique Cadenas
Feb 24, 2004·Molecular and Cellular Biochemistry·Marco Colombini
Aug 12, 2004·Free Radical Biology & Medicine·Martin D BrandNadeene Parker
Apr 6, 2005·Biochemistry. Biokhimii︠a︡·A Yu AndreyevA A Starkov
Aug 17, 2005·The International Journal of Biochemistry & Cell Biology·Petr Jezek, Lydie Hlavatá
Oct 26, 2005·Analytical Biochemistry·Christos D GeorgiouTheodore Sideris
Nov 26, 2005·Biochimica Et Biophysica Acta·John J Lemasters, Ekhson Holmuhamedov
May 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Johnathan C LaiC A Stein
Dec 1, 2006·American Journal of Physiology. Cell Physiology·Wenzhi TanMarco Colombini
Apr 10, 2007·Nature Cell Biology·Christopher P BainesJeffery D Molkentin
May 4, 2007·Biochemical and Biophysical Research Communications·Partha MukhopadhyayPál Pacher
May 8, 2007·Biophysical Journal·Wenzhi TanMarco Colombini
Jul 10, 2007·Biochimica Et Biophysica Acta·Wenzhi Tan, Marco Colombini
Dec 19, 2007·Sub-cellular Biochemistry·Zhenbo CaoNeil W Isaacs
Jan 4, 2008·The Journal of Biological Chemistry·Gundars GoldsteinsJari Koistinaho
Jun 5, 2008·The Journal of Biological Chemistry·Stefan Dröse, Ulrich Brandt
Jun 11, 2008·Biochemistry·Federica SinibaldiRoberto Santucci
Nov 19, 2008·Archives of Biochemistry and Biophysics·Ekhson Holmuhamedov, John J Lemasters
Feb 27, 2009·Bioinformatics·J VanlierD A Beard
Mar 4, 2009·The Journal of Biological Chemistry·Diane E HandyJoseph Loscalzo
Jun 11, 2009·Methods in Molecular Biology·Adrian J Lambert, Martin D Brand

❮ Previous
Next ❯

Citations

Jan 10, 2012·The Journal of Biological Chemistry·Ekhson L HolmuhamedovJohn J Lemasters
Jun 6, 2013·Human Molecular Genetics·Sarah PicklesChristine Vande Velde
Dec 17, 2010·Cancer Research·Eduardo N MaldonadoJohn J Lemasters
Dec 3, 2014·Biochimica Et Biophysica Acta·Debanjan TewariAmal Kanti Bera
Jan 29, 2014·Oncogene·L LeanzaI Szabo
Oct 16, 2012·Fertility and Sterility·Woo-Sung KwonMyung-Geol Pang
Nov 9, 2011·Biochimica Et Biophysica Acta·Kyle S McCommis, Christopher P Baines
Apr 23, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yang WangYunqing Cai
Jan 30, 2015·Frontiers in Physiology·Shivendra G TewariDaniel A Beard
Oct 4, 2014·Frontiers in Physiology·Bhawana AgarwalAmadou K S Camara
Nov 26, 2005·Biochimica Et Biophysica Acta·John J Lemasters, Ekhson Holmuhamedov
Dec 3, 2014·Biochimica Et Biophysica Acta·Varda Shoshan-BarmatzShambhoo Sharan Tripathi
Sep 19, 2019·Proceedings of the National Academy of Sciences of the United States of America·Colin H LipperPatricia A Jennings
Jul 18, 2017·Frontiers in Physiology·Amadou K S CamaraWai-Meng Kwok
Apr 24, 2018·Frontiers in Chemistry·Andrea MagrìVito De Pinto
Oct 13, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Larisa EmelyanovaArshad Jahangir
Dec 8, 2020·Frontiers in Cell and Developmental Biology·Camille DejosAnna Rita Cantelmo
Mar 23, 2021·FEBS Letters·Chaonan JinJean-François Dufour
Aug 24, 2021·Frontiers in Physiology·Barkha RaviGirdhar K Pandey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochimica Et Biophysica Acta
Wenzhi Tan, M Colombini
Proceedings of the National Academy of Sciences of the United States of America
Tatiana K RostovtsevaDan L Sackett
The Journal of Biological Chemistry
Tatiana K RostovtsevaS M Bezrukov
© 2021 Meta ULC. All rights reserved